循环内皮祖细胞与急性脑卒中及脑血管危险因素的相关研究

目的观察循环内皮祖细胞(EPC)数量和急性脑卒中及脑血管危险因素的关系。方法以CD133和KDR双阳性细胞作为EPC标记,对74例急性脑卒中患者(脑卒中组)和29例健康志愿者(正常对照组)的外周血淋巴细胞进行流式细胞分析,检测EPC数量;并检测血脂及高敏C反应蛋白(hs-CRP)。结果调整年龄、性别、收缩压及血清低密度脂蛋白胆固醇(LDL-C)后,与正常对照组比较,脑卒中组EPC数量明显减少,hs-CRP水平显著升高(P<0.01);EPC数量与年龄、收缩压、舒张压、血清总胆固醇、甘油三酯、LDL-C、hs-CRP均呈负相关(P<0.001),与性别及高密度脂蛋白胆固醇水平无关(P>0.36);多因素逐步回归分析显示,收缩压和LDL-C与EPC数量独立相关。结论循环EPC数量减少与急性脑卒中显著相关,收缩压与LDL-C水平是影响循环EPC数量的主要独立因素。

Clinical study on the association of circulating endothelial progenitor cells with stroke and cerebrovascular risk factors

Objective To investigate whether the number of the endothelial progenitor cells(EPC) might be associated with stroke and cerebrovascular risk factors.Methods EPC were identified as the CD133/KDR double positive cells by flow cytometry analysis.The number of circulating EPC was analyzed in 74 patients with acute stroke and in 29 healthy subjects.Serum lipids and high-sensitive CRP(hs-CRP) were measured with automated biochemical machine.Results After adjustment for age,sex,systolic blood pressure and serum low-density lipoprotein cholesterol,stroke patients had a significant lower number of EPC and a higher level of hs-CRP compared with healthy subjects(P<0.01).The number of circulating EPC was inversely(P<0.001) correlated with age,systolic and diastolic pressures,serum total cholesterol,triglyceride,(LDL-C,) and hs-CRP,but not with sex and high-density lipoprotein cholesterol(P>0.36).Multiple stepwise regression analysis showed that systolic blood pressure and LDL-C independently correlated with the EPC number, respectively.Conclusion The diminished circulating EPC was associated with acute stroke.Systolic blood pressure and LDL-C are significant and independent factors that may influence the number of circulating EPC.