冠心病患者胰岛素原水平、胰岛素抵抗与血管紧张素转换酶基因多态性关系的研究

目的　探讨冠心病患者血清胰岛素原 (PI)水平、胰岛素抵抗与血管紧张素转换酶 (ACE)基因插入 /缺失 (I/D)多态性的关系。方法　选择 66例经冠状动脉造影确诊的冠心病患者 ,分别采用单克隆抗体生物素 亲和素夹心放大酶联免疫分析法测定胰岛素原及真胰岛素 (TI)水平 ,并根据公式“TI×空腹血糖 (FBG) /2 2 5”计算HOMA指数。同时采用聚合酶链反应检测ACE基因I/D多态性。比较不同基因型间PI、HOMA指数的差异及不同PI、HOMA组间基因型和等位基因频率的分布。结果　不同基因型间PI及HOMA指数无显著差异。不同PI、HOMA组间基因型及等位基因频率分布亦无显著差异。结论　冠心病患者ACE基因I/D多态性与胰岛素原水平和胰岛素抵抗指标间无明显相关性。 2者在冠心病发病中可能分别发挥相对独立的作用

CLINICAL STUDY OF THE RELATIONSHIP BETWEEN ACE GENE POLYMORPHISM AND PROINSULIN, INSULIN RESISTANCE IN PATIENTS WITH CORONARY HEART DISEASE

Objective: To investigate the relationship between ACE gene p olymorphism and proinsulin, insulin resistance in patients with coronary heart d isease. Methods: 66 patients with coronary heart disease which was identified by coronary angiography were studied. Proinsulin (PI) and true i nsulin (TI) were detected by BA-ELISA and ACE I/D polymorphism by polymerase ch ain reactions(PCR). Insulin resistance was calculated by the homestasis model as sessment (HOMA) method. PI and HOMA levels were compared among different genotyp es by analysis of variance and the frequency of alleles and genotype in differen t PI and HOMA groups were analyzed by χ 2 test. Results: The re was no significant difference of PI and HOMA levels among different genotypes and there was also no significant difference in the frequency of allele and gen otype between different PI and HOMA groups. Conclusions: There was no association between ACE gene polymorphism and proinsulin, insulin resist ance in patients with coronary heart disease. They may play seperated role on th e development of coronary heart disease.