大鼠肠缺血再灌注损伤病理生理动态改变的实验研究

目的探讨大鼠肠缺血再灌注损伤的病理生理改变及其机理。方法采用夹闭大鼠肠系膜动脉制作肠缺血再灌注病理模型,研究大鼠肠缺血45 min再灌注后肠粘膜谷光甘肽(GSH)、氧化代谢产物 (MDA)抗氧化酶及肝肾功能的动态改变。结果肠缺血45 min后再灌注2、4、8 h,GSH明显减少;MDA 水平明显升高。肠粘膜CAT、SOD和GSH-PX活性未见明显改变。血清ALT和BUN明显升高。结论大鼠肠缺血再灌注可致肠粘膜严重氧化性损伤,并引起远隔器官(肝肾)功能受损,其损伤程度与再灌注时间呈现动态性改变。

The Pathophysiological Changes of Intestinal lschemia-reperfusion was Induced in Rats

Objectives To investigate the pathological changes of intestinal ischemia-reperfusion in rats. Methods The intestinal ischemia-reperfusion in rats with clamping superior mesenteric artery for 45 min followed by reperfusion. The levels of MDA, GSH, the activities of antioxidant enzymes in the gut mucosa including CAT, SOD, GSH-Px and serum ALT, AST, BUN, Cr were assayed at 2,4, 8, 12, 24 h after reperfusion. Results The levels of MDA and GSH in the gut mucosa increased and decreased significantly at 2 h of reperfusion. The activities of CAT, SOD and GSH-Px did not show significant changes in rats after intestinal ischemia-reperfusion. Serum ALT, AST, BUN and Cr increased significantly at 2 h of reperfusion. Conclusion This study showed that intestinal ischemia for 45 min followed by reperfusion diminished GSH level and increased MDA level and caused liver and renal reversible damage.