| Grp78和caspase-12在缺氧心肌细胞中的表达 |
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| 引用本文： | 李蕊君,何昆仑,李鑫,胡国梁,刘春蕾,王莉莉. Grp78和caspase-12在缺氧心肌细胞中的表达[J]. 军医进修学院学报, 2011, 32(3): 270-272 |
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| 作者姓名： | 李蕊君 何昆仑 李鑫 胡国梁 刘春蕾 王莉莉 |
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| 作者单位： | 1. 解放军总医院,南楼老年心内科,北京,100853
2. 军事医学科学院,毒物药物研究所,北京,100850 |
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| 基金项目： | 国家科技部国际科技合作重大专项课题 |
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| 摘 要： | 目的利用体外培养的心肌细胞缺氧模型,观察不同缺氧时间点Grp78和caspase-12在心肌细胞中的表达变化。方法将原代培养的大鼠心肌细胞,随机分为正常对照组,缺氧0.25h、0.5h、1h、1.5h、2h、4h、8h、12h、16h、24h组,通过TUNEL法检测缺氧心肌细胞凋亡,应用Westernblot法检测不同缺氧时间点Grp78和caspase-12在心肌细胞中的表达。结果与对照组相比,缺氧组细胞凋亡指数随缺氧时间延长逐渐升高;Grp78蛋白于缺氧0.25h开始表达,缺氧1h达到峰值;procaspase-12于缺氧0.5h表达开始增高,2h达到峰值;caspase-12于缺氧1h表达上升,4h达到峰值。结论缺氧激活了内质网应激反应,可引起心肌细胞凋亡。
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| 关 键 词： | 缺氧 内质网 肌细胞,心脏 Grp78 caspase12 |
Expression of glucose-regulated protein 78 and caspase-12 in hypoxic rat cardiomyocytes |
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| LI Rui-jun,HE Kun-lun,LI Xin,HU Guo-liang,LIU Chun-lei,WANG Li-li. Expression of glucose-regulated protein 78 and caspase-12 in hypoxic rat cardiomyocytes[J]. Academic Journal of Pla Postgraduate Medical School, 2011, 32(3): 270-272 |
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| Authors: | LI Rui-jun HE Kun-lun LI Xin HU Guo-liang LIU Chun-lei WANG Li-li |
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| Affiliation: | LI Rui-jun1,HE Kun-lun1,LI Xin1,HU Guo-liang1,LIU Chun-lei1,WANG Li-li2 1Department of Geriatric Cardiology,Chinese PLA General Hospital,Beijing 100853,China,2Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850 |
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| Abstract: | Objective To observe the expression of glucose-regulated protein 78 (Grp78) and caspase-12 in hypoxic rat cardiomyocytes.Methods Neonatal rat cardiomyocytes were randomly divided into control group and hypoxia 0.25h,0.5h,1h,1.5h,2h,4h,8h,12h,16h,24h groups.Apoptosis was assayed by TUNEL.Expression of Grp78 and caspase-12 was detected by Western blot at different time points.Results Compared with the control group,the apoptotic index increased with hypoxia time,the expression of Grp78 increased at 0.25h afte... |
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| Keywords: | Grp78 caspase12 |
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