灵菊七不同温度提取物抗氧化活性研究

目的:研究提取温度对灵菊七提取物总多酚、总黄酮含量及其抗氧化活性的影响;方法:采用不同温度(40-100℃)提取灵菊七药材,Folin-Ciocalteu及三氯化铝显色法测定灵菊七不同温度提取物的总多酚、总黄酮含量,DPPH、ABTS+.及磷钼酸铵三种抗氧化模型研究其抗氧化活性;结果:随着药材提取温度的升高,总多酚、总黄酮含量及抗氧化活性均显著升高(P<0.05),当提取温度达到100℃时,其总多酚、总黄酮含量及抗氧化活性与提取温度为90℃提取物无显著性差异(P>0.05)相关性分析表明总多酚、总黄酮含量与抗氧化活性显著相关(P<0.001);结论:提取温度对灵菊七提取物总多酚、总黄酮含量及抗氧化活性均有显著的影响。

Antioxidant potential of Gynura medica extracts obtained by different extraction temperatures

Objective：Research of the effect of extraction temperature on total phenols,total flavonols and antioxidant capacity of Gynura medica.Methods：Gynura medica was extracted at different temperature（40-100℃）,the Folin-Ciocalteu and aluminium choride colorimetry methods were used to determinate the content of total phenols and total flavonols;three antioxidant models including DPPH,ABTS＋· and phosphomolybdenum methods were selected to evaluate the antioxidant activity of Gynura medica.Results：The total phenols,total flavonols and antioxidant capacity of Gynura medica were all significantly increased（P〈0.05） with increasing the extraction temperature（from 40°C to 100°C）.While total phenols,total flavonols and antioxidant capacity were not significant different（P〉0.05） at the extraction temperature of 90°C and 100°C;the correlations revealed that the total phenols,total flavonols were all significantly correlated with the antioxidant activity（P〈0.001）.Conclusion：Extraction temperatures significantly influenced the total phenols,total flavonols and antioxidant capacity of Gynura medica.