肝动脉重建的小鼠原位肝移植模型建立 |
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引用本文: | 黄德荣,吴忠均,朱宇. 肝动脉重建的小鼠原位肝移植模型建立[J]. 中华实验外科杂志, 2006, 26(1): 931-932,插图7-4,封3. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.044 |
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作者姓名: | 黄德荣 吴忠均 朱宇 |
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作者单位: | 重庆医科大学附属第一医院肝胆外科,400016; |
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基金项目: | 国家自然科学基金资助项目重庆市卫生局科研资助项目 |
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摘 要: | 目的 建立稳定的肝动脉重建的小鼠原位肝移植模型.方法 以"双袖套法"小鼠肝移植为基础,将带腹主动脉的供肝肝动脉与受体腹主动脉行端侧吻合.共完成小鼠原位肝移植70例,并观察术后24 h、1周、1个月受体存活率,术后1个月肝功能及肝组织病理变化.同时设立假手术组对照.结果 受体术后24 h、1周、1个月存活率分别为94.3%、91.4%、85.7%.术后1个月肝功能ALT:(39.20±2.09)U/L AST:(75.60±3.24)U/L.与对照组比较,差异无统计学意义(P>0.05).病理显示肝组织结构良好.结论 该方法可稳定地建立肝动脉重建的小鼠原位肝移植模型.
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关 键 词: | 肝移植 肝动脉重建 模型,动物 |
Surgical experience of arterialized orthotopic liver transplantation model in mice |
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Abstract: | Objective To establish the arterialized liver transplantation model in mice. Methods On the basis of "two cuff" orthotopic liver transplantation in mice, graft aorta end-to-side anastomosis to recipient aorta was applied to reconstruct hepatic artery. Twenty-four h-, 1 week-, 1 month-postoperative survival rate, hepatic function, and pathological changes were observed. Sham operation group was set up. Results 94.3% ,91.4% and 85.7% of recipients survived after 48 h,one week and one month ,respec-tively. The liver functioned normally after one month postoperatively. Pathologically, the hepatic tissue had good structure. Conclusion It is an ideal mothods to establish the arterialized orthotopic liver transplanta-tion model in mice. |
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Keywords: | Liver transplantationHepatic rearterializationModel animal |
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