| Effects of pioglitazone on proliferation and differentiation of human preadipocytes |
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| 引用本文： | Jianying Zhou,Minjuan Ge,Yamei Wang,Haiwei Wu,Jie Shen,Xianghua Ma. Effects of pioglitazone on proliferation and differentiation of human preadipocytes[J]. 南京医科大学学报(自然科学版), 2007, 21(2): 99-103 |
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| 作者姓名： | Jianying Zhou Minjuan Ge Yamei Wang Haiwei Wu Jie Shen Xianghua Ma |
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| 作者单位： | [1]Department of Endocrine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; [2]Department of Endocrine, the First People＇s Hospital of Changzhou , Changzhou 213003, China [3]Staff Room of Etiological Agentmicrobiology ,Nanjing Medical University ,Nanjing 210029, China; [4]Gene Therapy Center,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029, China |
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| 摘 要： | Objective: To explore the effects of thiazolidinediones (TZDs) pioglitazone on proliferation and differentiation of human preadipocytes. Methods :Omental adipose tissue biopsies were obtained from 15 patients who were undergoing elective open-abdominal surgery. The primary culture and differentiated induction of human preadipocytes were performed, and the human preadipo-cytes were treated with pioglitazone at different concentrations at proper moments. Dynamic morphological changes of the human preadipocytes were observed, and their proliferation and differentiation were assessed with Colorimetric MTT Assay and Oil Red O Staining. Results:After 24 hours and 72 hours with pioglitazone, 0.1 μmol/L (μmol/ml) pioglitazone increased the MTT values of the human preadipocytes by 25.3% and 34.8% ,respectively(P ＜ 0.05), while 1 μ mol/L pioglitazone by 27.4% and 26.6%(P＜ 0.05), compared with the control group without pioglitazone. The human preadipocytes with pioglitazone cumulated more adipose in the endochylema than those without pioglitazone obviously. 0.1 μmol/L pioglitazone increased the differentiation degree of the human preadipocytes differentiated for 8-10 days by 44.81% and 1 μmol/L pioglitazone by 53.76%(P ＜ 0.05). Conclusion:Thiazolidinediones pioglitazone may significantly promote the proliferation and differentiation of the human omental preadipocytes.
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| 关 键 词： | 细胞增殖 前成脂肪细胞 形态学 动力学 |
| 收稿时间： | 2006-11-17 |
Effects of pioglitazone on proliferation and differentiation of human preadipocytes |
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| Jianying Zhou,Minjuan Ge,Yamei Wang,Haiwei Wu,Jie Shen and Xianghua Ma. Effects of pioglitazone on proliferation and differentiation of human preadipocytes[J]. Acta Universitatis Medicinalis Nanjing, 2007, 21(2): 99-103 |
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| Authors: | Jianying Zhou Minjuan Ge Yamei Wang Haiwei Wu Jie Shen Xianghua Ma |
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| Abstract: | Objective: To explore the effects of thiazolidinediones (TZDs) pioglitazone on proliferation and differentiation of human preadipocytes. Methods :Omental adipose tissue biopsies were obtained from 15 patients who were undergoing elective open-abdominal surgery. The primary culture and differentiated induction of human preadipocytes were performed, and the human preadipo-cytes were treated with pioglitazone at different concentrations at proper moments. Dynamic morphological changes of the human preadipocytes were observed, and their proliferation and differentiation were assessed with Colorimetric MTT Assay and Oil Red O Staining. Results:After 24 hours and 72 hours with pioglitazone, 0.1 μmol/L (μmol/ml) pioglitazone increased the MTT values of the human preadipocytes by 25.3% and 34.8% ,respectively(P ＜ 0.05), while 1 μ mol/L pioglitazone by 27.4% and 26.6%(P＜ 0.05), compared with the control group without pioglitazone. The human preadipocytes with pioglitazone cumulated more adipose in the endochylema than those without pioglitazone obviously. 0.1 μmol/L pioglitazone increased the differentiation degree of the human preadipocytes differentiated for 8-10 days by 44.81% and 1 μmol/L pioglitazone by 53.76%(P ＜ 0.05). Conclusion:Thiazolidinediones pioglitazone may significantly promote the proliferation and differentiation of the human omental preadipocytes. |
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| Keywords: | pioglitazone human preadipocytes proliferation and differentiation insulin sensitivity human differentiation proliferation pioglitazone promote degree control group increased values Results MTT Assay Dynamic morphological changes concentrations moments primary culture differentiated induction adipose tissue |
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