| Galectin-1在大肠癌细胞系中的表达及意义 |
| |
| 引用本文： | 吕志强,朱曦龄,郭晓东,马立芝. Galectin-1在大肠癌细胞系中的表达及意义[J]. 中国临床保健杂志, 2012, 15(4): 394-396 |
| |
| 作者姓名： | 吕志强 朱曦龄 郭晓东 马立芝 |
| |
| 作者单位： | 武警总医院急救医学中心,北京,100039 |
| |
| 摘 要： | 目的探讨Galectin-1在大肠癌细胞系中的表达情况,以及与大肠癌侵袭、转移的关系。方法用Real time-RT PCR法和免疫组织化学法分析Galectin-1mRNA及蛋白在5种大肠癌细胞系的表达情况;用Boyden小室法比较5种大肠癌细胞系的侵袭转移能力。结果 Galectin-1在大肠癌转移灶来源的细胞系中的表达高于大肠癌原发灶来源的细胞系(P<0.01);Boyden小室的结果显示大肠癌转移灶来源的细胞系的侵袭,转移能力高于大肠癌原发灶来源的细胞系。结论 Galectin-1的高表达可能与大肠癌的侵袭转移有一定关系。
|
| 关 键 词： | 结直肠肿瘤 凝集素类 肿瘤转移 肿瘤侵润 |
The expression of Galectin-1 in human colorectal carcinoma cells and its significance |
| |
| LV Zhi-qiang , ZHU Xi-ling , GUO Xiao-dong , MA Li-zhi. The expression of Galectin-1 in human colorectal carcinoma cells and its significance[J]. Chinese Journal of Clinical Healthcare, 2012, 15(4): 394-396 |
| |
| Authors: | LV Zhi-qiang ZHU Xi-ling GUO Xiao-dong MA Li-zhi |
| |
| Affiliation: | (Emergency Center,General Hospital of Armed Police Forces,Beijing 100039,China) |
| |
| Abstract: | Objective To explore the expression of Galectin-1 gene in colorectal cancer cell lines and its correlation with tumor metastasis.Methods Expression of Galectin-1 in colorectal cancer cell lines was detected by Real time-RT PCR and imunohistochemistry.In vitro invasiveness was determined by Boyden chamber assay.The correlation of Galectin-1 expression with the invasive ability was also analyzed.Results Galectin-1 was highly expressed in LoVo and SW620.In vitro,cell invasivion demonstrated that LoVo and SW620 had a higher invasive ability than LS174T,SW480 or HT29.Conclusion Galectin-1 may play an important role in invasion and metastasis of colorectal cancer. |
| |
| Keywords: | Colorectal neoplasms Agglutinins Neoplasm metastasis Neoplasm invasiveness |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
