The significance of post-mortem vitreous calcium concentration in forensic practice |
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| Affiliation: | 1. Institute of Forensic Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia;2. Institute of Forensic Medicine, School of Medicine, University of Priština (Kosovska Mitrovica), Serbia;3. Institute for Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia;1. Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India;2. Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi, India;3. Department of Ocular Pharmacology and Pharmacy, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India;1. Dept. of Forensic Medicine, University College of Medical Sciences, Delhi, India;2. Dept. of Public Health Dentistry, Kalka Dental College, Meerut, U.P., India;3. Dept. of Biochemistry, University College of Medical Sciences, Delhi, India;1. Forensic DNA Typing Laboratory, Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan;2. Forensic DNA Typing Laboratory, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan;3. Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan;1. Department of Legal Medicine, Osaka City University, Medical School, Asahimachi 1-4-3, Abenoku, Osaka 545-8585, Japan;2. Department of Legal Medicine, Goethe-University Frankfurt, Medical School, Kennedyallee 104, 60596 Frankfurt am Main, Germany |
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| Abstract: | Calcium, as one of the main extracellular ions, maintains a key role in numerous biologic functions. For forensic purposes, it was analyzed mostly for estimation of postmortem interval (PMI). We have designed our experiment with the concept that the repetitive withdrawal of vitreous humor (VH) might clarify the postmortem metabolism of calcium in greater detail to estimate the PMI. Accordingly, 248 samples of VH from 31 autopsy cases were evaluated over three years; samples (0,1 mL of VH) were taken and analyzed at equal time intervals after death—every three hours until 24 h after death. Each sample was centrifuged and analyzed using the ARCHITECT C SYSTEM 8000. Moreover, functional relationship between PMI and calcium concentration was established: PMI (hours) = [Ca2+] × 13.696–7.843. Although the concentration of calcium in VH in the analyzed group increases with time, the coefficient of variation for the regression (CVreg = 46.8%) indicates that this correlation is not so strong, meaning that the level of predictiveness of calcium for estimation of time since death is poor when is not used in combination with other relevant substances. |
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| Keywords: | Post-mortem interval Vitreous humor Calcium Autopsy |
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