A genomic exploration of 15 autosomal STR loci for establishment of a DNA profile database of the population of Himachal Pradesh |
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| Affiliation: | 1. DNA Fingerprinting Division, Central Forensic Science Laboratory, CBI, CGO Complex, New Delhi 110003, India;2. DNA Fingerprinting Unit, State Forensic Science Laboratory, Department of Home (Police), Govt. of MP, Sagar 470001, India;3. DNA Division, State Forensic Science Laboratory, Jaipur, Rajasthan, India;4. Cytogenetics Laboratory, Dept of Zoology, Banaras Hindu University, Varanasi 221005, UP, India;1. Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China;2. Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Research Institute of Criminal Science and Technology, Shanghai 200083, China;3. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China;1. Baotou Medical College, School of Basic Medicine, Baotou 014060, Inner Mongolia Autonomous Region, China;2. Academy of Forensic Science-Ministry of Justice-China, Forensic Genetic, Shanghai 200063, China;3. Sichuan University, Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Chengdu 610064, Sichuan Province, China;4. Department of Forensic Medicine, Zunyi Medical University, Zunyi 563099, Guizhou, China;5. Department of Forensic Medicine, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China;1. DNA Fingerprinting Unit, State Forensic Science Laboratory, Department of Home (Police), Govt. of MP, Sagar 470001, India;2. DNA Division, State Forensic Science Laboratory, Rajasthan, Jaipur 302016, India;3. Institute of Forensic Science and Criminology, Bundelkhand University, Jhansi 284128, UP, India;1. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, PR China;2. Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou 510080, PR China;1. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, PR China;2. Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou 510080, PR China;3. The Second Clinical Medical school (Zhujiang Hospital), Southern Medical University, Guangzhou 510280, PR China;1. Department of General Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, China;2. Shenzhen Sixth Hospital Affiliated to Guangdong Medical College, China;3. Department of Radiology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, China;4. Department of Orthodontics, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, China |
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| Abstract: | In order to create an autosomal STR loci population database for Himachal Pradesh, 259 blood samples were taken from people residing in various regions of the state and AmpFlSTR® Identifiler® Plus PCR amplification kit was used for evaluation of 15 autosomal STR markers. A total of 149 alleles were investigated in this study with a mean allele number of 9.933 per locus. The locus D2S1338 was most informative in our data, as it had the highest discrimination power (PD-0.967) and the highest polymorphic information content (PIC-0.86). The matching probability and typical paternity index for all the studied loci were observed as 2.9x10-18 and 4.7x105, respectively. Discrimination power (CPD) and exclusion power (CPE) for all the studied loci were observed as 1 and 0.999998. |
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| Keywords: | Autosomal STRs Forensic DNA typing Population database Himachal Pradesh India |
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