Identification of miRNA signatures in serum exosomes as a potential biomarker after radiotherapy treatment in glioma patients |
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| Institution: | 1. Department of Chemistry, Washington University, St. Louis, Missouri;2. Department of Radiology, Washington University, St. Louis, Missouri;3. Department of Radiation Oncology, Washington University, St. Louis, Missouri;4. Department of Neurosurgery, Washington University, St. Louis, Missouri;5. Department of Internal Medicine, Washington University, St. Louis, Missouri;7. Alvin J. Siteman Cancer Center, Washington University, St. Louis, Missouri;1. Department of Science and Education, Jiangxi Key Laboratory of Translational Cancer Research, Jiangxi Cancer Hospital, Nanchang, Jiangxi, 330029, PR China;2. Department of Pharmacy, Jiangxi Provincial Children’s Hospital, Nanchang, Jiangxi, 330029, PR China;3. Department of Radiation Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi, 330029, PR China;4. Department of Pharmacy, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330029, PR China;5. Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, 410008, China;1. The Second Affiliated Hospital of Soochow University, San Xiang Road No. 1055, Suzhou, Jiangsu, China;2. Institute of Radiotherapy & Oncology, Soochow University, Suzhou, Jiangsu, China;3. Suzhou Key Laboratory for Radiation Oncology, Suzhou, Jiangsu, China;4. Institute of Neuroscience, Soochow University, Ren Ai Road No. 199, Suzhou, Jiangsu, China;5. Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, China;1. Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China;2. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, China;1. Department of Medical Genetics, Yeditepe University Medical School, Istanbul, Turkey;2. Department of Biotechnology, Institute of Science, Yeditepe University, Istanbul, Turkey;3. Department of Nanoscience and Nanoengineering, Institute of Science Ataturk University, Erzurum, Turkey;4. Department of Neurosurgery, Yeditepe University Medical School, Yeditepe University, Istanbul, Turkey;5. Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey;6. Department of Biochemistry, Yeditepe University Medical School, Istanbul, Turkey |
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| Abstract: | Despite development in therapies, the high recurrence and low positivity of biomarkers for diagnosis still result in glioma with high mortality. In this study, we aimed to identify a potential miRNA signature to evaluate the effect of radiotherapy in glioma patients. MicroRNA (miRNA) sequencing was performed on miRNAs isolated from serum exosomes in a cohort of glioma patients before and after radiotherapy. A total of 18 up-regulated differentially expressed (DE) miRNAs and 16 down-regulated DE miRNAs were identified. Subsequently, the target genes of DE miRNAs were predicted based on multiple miRNA-target databases. Further, it was indicated that these targets were primarily involved in metabolic process, p53 signaling pathway and cancer pathways, suggesting that these miRNAs play a crucial role in glioma by regulating targets and affect the occurrence and development of the disease. In general, this study presented the variation of miRNAs in blood exosomes before and after radiotherapy. It can not only be helpful for the diagnosis of glioma, but also find new candidate biomarkers for monitoring the condition and evaluating the efficacy of radiotherapy in glioma. It provides a new idea for the diagnosis, treatment and prognosis evaluation of glioma. |
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