ob基因型对小鼠早期发育的影响

目的 探讨ｏｂ基因对早期发育的影响及其在发育早期的显性程度。方法 用聚合酶链反应和限制性内切酶法检测Ｃ５７ＢＬ／６Ｊ ｏｂ小鼠的３种基因型：野生型（＋／＋）、杂合体型（ｏｂ／＋）及突变型（ｏｂ／ｏｂ）。记录小鼠出生４ｄ～２个月的体重。比较出生后７、１４和２４ｄ小鼠的腹股沟白色脂肪（ＷＡＴ）和背肩岬的褐色脂肪（ＢＡＴ）湿重。结果 小鼠出生２周后,ｏｂ／ｏｂ小鼠体重快速增长,７周时已是＋／＋和ｏｂ／＋

Effects of ob genotype on early development in mice

OBJECTIVE: To study the effects and the extent of dominance of ob gene on early development in mice. METHODS: ob genotypes, wild type (+/+), hybrid type (ob/+) and mutant type (ob/ob), were detected by polymerase chain reaction and restrict endonuclease technique. Body weight of mice was recorded daily for the first seventh weeks of life. Their wet weight of white adipose tissue (WAT) and brown adipose tissue (BAT) was compared at the 7th, 14th and 24th days after birth. RESULTS: Body weight of ob/ob mice increased rapidly two weeks after birth and reached two times of that of +/+ and ob/+ mice seven weeks after birth. WAT of ob/ob mice was significantly higher than that of +/+ and ob/+ mice 24 days after birth. Effect of ob genotype on BAT was earlier than that on WAT. Body weight and WAT of ob/+ mice ranged between those of +/+ and ob/ob mice. Dominance index, reflecting the effect of ob gene on body weight, ranged from 1.0 - 0.5 within the first two weeks after birth, appearing co-dominance. And, dominance index approximated to 0.0 six weeks after birth, appearing almost complete recession. CONCLUSION: Effect of ob gene on body weight and WAT initiated two weeks after birth and increased with age, developing gradually from co-dominance to recession in their early life.