| 阴性突变Rac-N17基因转染对人脐静脉内皮细胞凋亡的影响 |
| |
| 引用本文： | 杜建时,赵晴,宋天琪.阴性突变Rac-N17基因转染对人脐静脉内皮细胞凋亡的影响[J].中国实验诊断学,2006,10(12):1428-1430. |
| |
| 作者姓名： | 杜建时 赵晴 宋天琪 |
| |
| 作者单位： | 吉林大学中日联谊医院,吉林,长春,130033 |
| |
| 摘 要： | 目的 探讨基因转染对内皮细胞凋亡的影响及其相关机制。方法 采用凝血酶诱导法诱导人脐静脉内皮细胞株（HUVECs）凋亡，检测细胞凋亡情况及caspase-3活性。结果 结果显示，凝血酶诱导48 h后，HUVECs细胞浆内颗粒和空泡增多，细胞凋亡率明显增高，caspase-3活性明显增加，而加入RacN17干预后，细胞凋亡率明显降低、caspase-3活性明显下降。结论 Rac-N17基因转染能防止细胞的凋亡与衰老，保护血管内皮细胞。
|
| 关 键 词： | 人脐静脉内皮细胞 凋亡 |
| 文章编号： | 1007-4287(2006)12-1428-03 |
| 收稿时间： | 10 28 2006 12:00AM |
| 修稿时间： | 2006年10月28 |
Effection of Rac-N17 infection on HUVECs apoptosis |
| |
| DU Jian-shi,ZHAO Qing,SONG Tian-qi.Effection of Rac-N17 infection on HUVECs apoptosis[J].Chinese Journal of Laboratory Diagnosis,2006,10(12):1428-1430. |
| |
| Authors: | DU Jian-shi ZHAO Qing SONG Tian-qi |
| |
| Institution: | China-Japan Union Hospital of Jilin University, Changchun 130033, China |
| |
| Abstract: | Objective To investigate the effect of Rac-N17 on the process of the human umbilical vein endothelial cell(HUVEC) apoptosis.Methods Inducce HUVECs apoptosis and use Rac-N17 infection.to interfere it.Meanwhile,apoptosis rate and caspase-3 activity were investigated.Results The results indicated that in model group the HUVECs became enlarged and flattened,apoptosis rate and caspase-3 activity increased,while RacN17 group have a decreased apoptosis rate and caspase-3 activity,suggested that endothelial senescence could be decreased by Rac-N17 infection.Conclusion The cell apoptosis can be prevented and the vein endothelial cell can be proteeted by Rac-N17 infection. |
| |
| Keywords: | Rac-N17 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
