Effects of CS–US interval modification on diminution of the unconditioned response in electrodermal classical conditioning

The purpose of this experiment was to study whether interstimulus interval (ISI) modification differentially affects the amplitude of the unconditioned response (UR amp.). Seventy-five volunteer subjects received discrimination training with an interval between conditioned stimulus (CS) and unconditioned stimulus (US; aversive white noise) of 8 s. After the discrimination training phase, subjects exhibiting discriminative control were randomized into three groups that differed according to the ISI, of 1, 5 or 8 s. The subjects of each group were then tested with five presentations of CS+/US. The results demonstrate that the UR showed a greater amplitude when an ISI of 8 s was used than with ISIs of 1 or 5 s. No significant differences were found between the UR amp. of the 1- and 5-s ISI groups. These findings are discussed as a result of a hypothetical experimentally induced blend of the anticipatory CRs with the UR.