震颤宁治疗帕金森病的实验研究

目的]探讨中药襄颤宁治疗帕金森氏病(PD)的药效机理。方法]采用6-羟多巴胺(6-OHDA)纹状体两点注射法复制PD模型,观察震颤宁对大鼠旋转行为、纹状体及血液中多巴胺(DA)和高香草酸(HVA)、过氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)含量的影响。结果]高剂量震颤宁可改善PD大鼠旋转行为,使旋转圈数较治疗前减少(P<0.05);提高大鼠全血GSH舍量(P<0.05),使GSH-PX活性显著增高(P<0.01),血清MDA含量显著降低:右侧纹状体GSH含量显著增高(P<0.01),MDA含量显著降低(P<0.01),并可增加纹状体DA含量,降低HVA/DA比值。低剂量震颤宁对PD大鼠旋转行为无明显改善(P>0.05);可降低血清MDA含量(P<0.01),而对GSH-PX活性无明显改善;并可使大鼠纹状体GSH含量增加,而对MDA含量无明显影响,对DA、HVA/DA亦无显著影响。结论]震颤宁可能通过改善机体及纹状体的氧化应激状态和保护神经元,间接补充纹状体DA含量而改善PD症状,并呈剂量效应关系。

Experimental Studies on Zhenchan Ning for Parkinson's Disease

Objective ] To investigate the mechanism of Zhenchan Ning (ZN) for Parkinson's Disease ( PD). Methods] Rat models of PD were established by striatal infusion of 6-hydroxy dopamine (6-OHDA) . Effect of ZN on rat rotating behavior was observed, and the contents of dopamine ( DA), homovanillic acid (HVA), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GSH-PX) in blood and corpus striatum were also detected. Results] As compared with those before treatment, high-dose ZN could improve the rotating behavior and decrease the rotating frequency ( P < 0.05), increase GSH content in blood ( P < 0.05) and corpus striatum (P < 0.01) and serum GSH-PX activity (P < 0.01), decrease MDA content in serum and corpus striatum (P<0.01), increase DA content in corpus striatum and reduce the HVA/DA ratio. Low-dose ZN exerted no effect on the rotating behavior, GSH-PX activity, MDA content in striatum, DA and HVA/DA ( P > 0.05), but could decrease serum MDA content (P < 0.01) and increase striatal GSH content. Conclusion] ZN can improve the symptoms of PD in a dose-effect manner. Its mechanism may be related to the improvement of bodily and striatal oxidization stress, the protection of neurons and the indirect supplement of striatal DA content.