| 荧光光谱法水相测定乳制品中三聚氰胺 |
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| 引用本文： | 严成艳,吕昌银,冯伟,程健琳,陈兴会,贺元文,宁玲. 荧光光谱法水相测定乳制品中三聚氰胺[J]. 中国卫生检验杂志, 2010, 0(1) |
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| 作者姓名： | 严成艳 吕昌银 冯伟 程健琳 陈兴会 贺元文 宁玲 |
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| 作者单位： | 南华大学公共卫生学院卫生化学检测教研室; |
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| 基金项目： | 湖南省科技计划项目(编号2009FJ3067)资助 |
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| 摘 要： | 目的:建立一种荧光光谱法水相测定三聚氰胺的新方法;应用酸效应对型体分布的影响,研究了反应机理。方法:在水介质中,三聚氰胺与荧光素钠缔合,使体系的荧光强度增强,从而建立三聚氰胺的测定新方法。结果:在实验最适条件下,在4.8×10-3~90μg/m l、1.6×10-2~280μg/m l范围内,体系荧光强度改变值(ΔF)与三聚氰胺的浓度分别呈现良好的线性关系,线性回归方程分别为ΔF=2660.3ρ+36.67(r=0.9992)、ΔF=768.92ρ+160.66(r=0.9996),检出限分别为1.4、5 ng/m l。样品加标回收率为95.7%~110.0%,RSD小于0.47%。结论:方法灵敏度高、精密度好、操作简便、费用低,用于乳制品中三聚氰胺的测定,结果满意。
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| 关 键 词： | 三聚氰胺 荧光素钠 荧光光谱法 水相 酸效应 |
Determination of melamine in dairy by spectrofluorometric method in water phase |
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| YAN Cheng-yan,LV Chang-yin,FENG Wei,CHENG Jian-lin,CHEN Xing-hui,HE Yuan-wen,NI Ling. Determination of melamine in dairy by spectrofluorometric method in water phase[J]. Chinses Journal of Health Laboratory Technology, 2010, 0(1) |
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| Authors: | YAN Cheng-yan LV Chang-yin FENG Wei CHENG Jian-lin CHEN Xing-hui HE Yuan-wen NI Ling |
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| Affiliation: | YAN Cheng-yan,LV Chang-yin*,FENG Wei,CHENG Jian-lin,CHEN Xing-hui,HE Yuan-wen,NI Ling (Department of Health Laboratory Technology,School of Public Health,University of South China,Hengyang 421001,China) |
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| Abstract: | Objective:To set up a novel spectrofluorometric method for the determination of melamine in water phase and study the reaction mechanism applying the influence of the acid effect on the species distribution.Methods:In water phase,melamine and fluorescein sodium formed the ion-association complex which resulted in the enhancement of fluorescence intensity.Based on this phenomenon,we had established a new method for the determination of trace melamine.Results:Under the optimum experimental conditions,it was f... |
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| Keywords: | Melamine Fluorescein sodium Spectrofluorometric method Water phase Acid effect |
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