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| 富氢液对大鼠全脑缺血再灌注损伤的影响 | |
| 引用本文： | 惠康丽,韩云飞,周玉弟,李丹,苗晓蕾,嵇晴,封小美,孙学军,段满林.富氢液对大鼠全脑缺血再灌注损伤的影响[J].医学研究生学报,2011,24(6):573-577. |
| 作者姓名： | 惠康丽 韩云飞 周玉弟 李丹 苗晓蕾 嵇晴 封小美 孙学军 段满林 |
| 作者单位： | 1. 221002,徐州,徐州医学院/江苏省麻醉学重点实验室;210002,南京,南京军区南京总医院麻醉科 2. 南京军区南京总医院训练中心,南京,210002 3. 南京军区南京总医院麻醉科,南京,210002 4. 徐州医学院/江苏省麻醉学重点实验室,徐州,221002 5. 上海交通大学附属瑞金医院麻醉科,上海,200025 6. 第二军医大学潜水医学系,上海,200433 |
| 基金项目： | 南京军区医药卫生科研基金 |
| 摘 要： | 目的脑组织缺血缺氧后,过量自由基产生,破坏组织细胞,形成再灌注损伤。最近,学者研究报道氢气溶解在液体中可选择性中和培养细胞中的羟自由基和亚硝酸阴离子。文中观察腹腔注射富氢液对大鼠暂时性全脑缺血再灌注损伤的影响。方法雄性SD大鼠90只,建立四血管阻塞全脑缺血(15 min)再灌注损伤模型,分为3组:假手术组(n=30),模型组(再灌注同时腹腔注射等渗盐水5ml/kg,n=30)和治疗组(再灌注同时腹腔注射0.6mmol/L富氢液5ml/kg,n=30),检测各组再灌注24 h海马丙二醛(malondialdehyde,MDA)浓度、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)浓度、核因子-κB(nu-clear factor-kappaB,NF-κB)免疫组化阳性细胞数和活化caspase-3的表达水平以及再灌注72 h海马CA1区HE染色未损伤锥体细胞数。结果与模型组相比,治疗组海马MDA浓度、TNF-α浓度和NF-κB阳性细胞数活均显著下降(P<0.01);活化caspase-3蛋白表达显著降低(P<0.05);海马CA1区锥体细胞数显著增多(P<0.01)。结论腹腔注射富氢液能够有效减轻全脑缺血(15 min)后再灌注时大鼠脑的氧化应激和炎症反应,进而缓减再灌注损伤。 |
| 关 键 词： | 富氢液 氧化应激 全脑缺血 再灌注 caspase-3 |
Hydrogen-rich saline reduces the injury from transient global cerebral ischemia-reperfusion in rats |
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| HUI Kang-li,HAN Yun-fei,ZHOU Yu-di,LI Dan,MIAO Xiao-lei,JI Qing,FENG Xiao-mei,SUN Xue-jun,DUAN Man-lin.Hydrogen-rich saline reduces the injury from transient global cerebral ischemia-reperfusion in rats[J].Bulletin of Medical Postgraduate,2011,24(6):573-577. | |
| Authors: | HUI Kang-li HAN Yun-fei ZHOU Yu-di LI Dan MIAO Xiao-lei JI Qing FENG Xiao-mei SUN Xue-jun DUAN Man-lin |
| Affiliation: | HUI Kang-li1,2,HAN Yun-fei3,ZHOU Yu-di2,LI Dan1,MIAO Xiao-lei2,JI Qing2,FENG Xiao-mei4,SUN Xue-jun5,DUAN Man-lin2(1.Key Laboratory of Anesthesiology of Jiangsu Province,Xuzhou Medical College,Xuzhou 221002,Jiangsu,China,2.Department of Anesthesiology,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,3.Training Center for Medical Professionals,4.Department of Anesthesiology,Ruijin Hospita... |
| Abstract: | Objective After hypoxia-ischemia,brain tissues generate excessive free radicals which will damage cells and tissues.Recently,it was reported that H2 dissolved in the medium could selectively reduce hydroxyl radical(OH-) and peroxynitrite(ONOO-) in cultured cells.Our research aims to observe the effect of intraperitoneal injection of hydrogen-rich saline on transient global cerebral ischemia-reperfusion injury in ratsh.Methods Models of global cerebral ischemia(15 min) and reperfusion with a four-vessel occl... |
| Keywords: | caspase-3 |
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