| 荧光实时定量PCR检测前列腺癌抗原3评分方法的建立 |
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| 引用本文： | 罗冰,王卫萍,刘明杰,王艾丽.荧光实时定量PCR检测前列腺癌抗原3评分方法的建立[J].医学研究生学报,2011,24(6). |
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| 作者姓名： | 罗冰 王卫萍 刘明杰 王艾丽 |
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| 作者单位： | 1. 南京军区南京总医院解放军临床检验医学研究所,南京,210002
2. 蚌埠医学院06生物科学系,蚌埠,233030 |
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| 基金项目： | 南京军区南京总医院青年基金 |
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| 摘 要： | 目的 前列腺癌抗原3(prostate cancer antigen 3,PCA3)基因表达具有前列腺癌特异性,文中建立荧光实时定量PCR(FQ-RT-PCR)检测PCA3评分的方法.方法 分别以LNCap和PC-3细胞株为阳性和阴性对照,用TaqMan探针建立检测PCA3和前列腺特异性抗原(prostate specific antigen,PSA) mRNA的荧光实时定量PCR方法,评价特异性、重复性和计算扩增效率,建立PCA3评分计算公式.结果 检测PCA3和PSA mRNA的批内变异系数分别为1.19%、1.09%、0.84%和0.06%、0.71%、0.79%,批间变异系数分别为1.03%和0.56%;扩增效率分别为0.98和1.00;以2-ΔCt表示PCA3评分.结论 荧光实时定量PCR检测PCA3评分方法的特异性好,重复性高.
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| 关 键 词： | 前列腺癌抗原3 前列腺特异性抗原 前列腺癌 荧光实时定量PCR |
Establishment of a real-time fluorescence quantitative polymerase chain reaction method for determining the PCA3 score |
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| LUO Bing,WANG Wei-ping,LIU Ming-jie,WANG Ai-li.Establishment of a real-time fluorescence quantitative polymerase chain reaction method for determining the PCA3 score[J].Bulletin of Medical Postgraduate,2011,24(6). |
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| Authors: | LUO Bing WANG Wei-ping LIU Ming-jie WANG Ai-li |
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| Institution: | LUO Bing1,WANG Wei-ping1,LIU Ming-jie2,WANG Ai-li1 (1.PLA Reseach Institute of Clinical Laboratory Medicine,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,Jiangsu,China,2.Department of Biology,Bengbu Medical College,Bengbu 233030,Anhui,China) |
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| Abstract: | Objective Prostate cancer gene 3(PCA 3) is specific for prostate cancer.This paper is to establish a real-time fluorescence quantitative polymerase chain reaction(FQ-RT-PCR) method for determining the PCA3 score.Methods LNCap and PC-3 cell lines were used as positive and negative controls,respectively,and a real-time fluorescence quantitative PCR method was established with the TaqMan probe for the detection of PCA3 and PSA mRNA,evaluate its specificity,reproducibility and amplification efficiency,and then ... |
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| Keywords: | Prostate cancer antigen 3 Prostate specific antigen Prostate cancer Real-time fluorescence quantitative polymerase chain reaction |
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