若干因素对戊二醛含量测定及稳定性影响的试验研究

为了解戊二醛配方中常用配伍成分对其含量测定和稳定性的影响,采用理化分析方法进行了实验室研究。结果,不加任何配伍成分的纯品戊二醛,其含量在18~25 g/L范围,用羟胺法测定误差范围在1.19%~2.24%。在含量为22.39 g/L戊二醛溶液中分别加入3.0 g/L与5.0 g/L亚硝酸钠,戊二醛溶液的pH值由3.93增加为4.84与5.00;戊二醛含量由22.39 g/L下降为22.26 g/L和22.01 g/L。在含量为21.88 g/L戊二醛溶液中分别加入1.0~3.0 g/L碳酸氢钠,其pH值由原来的3.93增加到7.37~7.80;戊二醛含量由原来的21.88 g/L下降到21.07~19.91 g/L。在含有碳酸氢钠和亚硝酸钠的戊二醛溶液中加入2.5 g/L脂肪醇聚氧乙烯醚,于室温下储存28 d,戊二醛下降率为3.70%;不加者下降率为5.67%。结论,用羟胺法测定戊二醛含量,随戊二醛溶液pH值变化,测定结果也会发生改变。戊二醛溶液中加入碳酸氢钠或亚硝酸钠,其戊二醛测定值下降。戊二醛溶液中加入脂肪醇聚氧乙烯醚,其稳定性有所加强。

EXPERIMENTAL STUDY ON INFLUENCE OF SOME FACTORS ON GLUTARALDEHYDE CONTENT MEASUREMENT AND STABILITY

In order to know the influence of common adjuvant ingredients of glutaraldehyde fomulas on glutaraldehyde content measurement and stability,physicochemical analysis was used to carry out laboratory study.Results: The glutaraldehyde content of pure glutaraldehyde without any adjuvant ingredient ranged 18～25 g/L and the error range was 1.19%～2.24% measured by hydroxy amine method.By addition of sodium nitrite 3.0 g/L and 5.0 g/L to the solution containing glutaraldehyde 22.39 g/L,the pH values of the glutaraldehyde solutions increased from 3.93 to 4.84 and 5.00 respectively and glutaraldehyde contents decreased from 22.39 g/L to 22.26 g/L and 22.01 g/L respectively.By addition of sodium bicarbonate 1.0～3.0 g/L to the solution containing glutaraldehyde 21.88 g/L,the pH values increased from original 3.93 to 7.37～7.80 and the glutaraldehyde content decreased from original 21.88 g/L to 21.07～19.91 g/L respectively.By addition of polyoxyethylene alcohol 2.5 g/L to glutaraldehyde solution containing sodium bicarbonate and sodium nitrite,followed by storing at room temperature for 28 days,the glutaraldehyde content decreased by 3.70%,while the glutaraldehyde content of the solution without addition of polyoxyethylene alcohol decreased by 5.67%.Conclusions: When hydroxy amine method was used for measuring glutaraldehyde content,the result of measurement changed with change in pH value of glutaraldehyde solution.Addition of sodium bicarbonate or sodium nitrite to glutaraldehyde solution caused decrease in measured value of glutaraldehyde.Addition of polyoxyethylene alcohol to glutaraldehyde solution enhanced its stability.