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| 人源性exCAR原核表达蛋白体外阻断腺病毒感染实验研究 | |
| 引用本文： | 张克斌,周小波,光丽霞,周世文,何晓梅,盛哈蕾,钱桂生.人源性exCAR原核表达蛋白体外阻断腺病毒感染实验研究[J].重庆医学,2009,38(19). |
| 作者姓名： | 张克斌 周小波 光丽霞 周世文 何晓梅 盛哈蕾 钱桂生 |
| 作者单位： | 1. 第三军医大学新桥医院医学实验技术中心,重庆,400037 2. 第三军医大学新桥医院心血管疾病研究所,重庆,400037 3. 第三军医大学新桥医院呼吸内科研究所,重庆,400037 |
| 基金项目： | 重庆市自然科学基金资助项目 |
| 摘 要： | 目的 在培养乳鼠心肌细胞上检测人源性柯萨奇病毒-腺病毒受体胞外区(exCAR)大肠杆菌原核表达蛋白阻断腺病毒感染的活性.方法 纯化人源性exCAR表达蛋白并通过透析复性,进一步体外实验,在腺病毒感染乳鼠心肌细胞模型上检测表达蛋白阻断腺病毒感染效率.结果 获得纯化表达蛋白exCAR,经复性后,在体外实验中检测到其阻断腺病毒感染心肌细胞效应,其终浓度为500ng/mL时,获得最高阻断效率约75%.结论 本研究获得了具有生物活性的exCAR表达蛋白,为腺病毒感染防治提供了新的思路和基础. |
| 关 键 词： | 腺病毒 柯萨奇-腺病毒受体 胞外区 蛋白纯化与复性 阻断效应 |
In vitro experimental study of blocking effect on adenovirus infection of prokaryotic expressed protein of human exCAR |
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| ZHANG Ke-bin,ZHOU Xiao-bo,GUANG Li-xia,et al..In vitro experimental study of blocking effect on adenovirus infection of prokaryotic expressed protein of human exCAR[J].Chongqing Medical Journal,2009,38(19). | |
| Authors: | ZHANG Ke-bin ZHOU Xiao-bo GUANG Li-xia |
| Institution: | ZHANG Ke-bin1,ZHOU Xiao-bo2,GUANG Li-xia1,et al.(1.Center of Medical Experiment , Technology,2.Institute of Cardiac Vascular Diseases,3.Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China) |
| Abstract: | Objective To test the blocking effect on adenovirus infection of the expressed protein of extra-cellular region of human Coxsackie-adenovirus receptor in neonatal rat cardiac myocytes.Methods The human exCAR protein was expressed in E.coli expression system pET-28a(+)/BL21,and the expression protein was purified and renatured by dialysis,and then the blocking effect on adenovirus infection to neonatal rat cardiac myocytes was measured.Results The purify expressed recombinant exCAR protein was obtained and r... |
| Keywords: | adenovirus coxsackie-adenovirus receptor extra-cellular region protein purification and renature blocking effect |
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