血浆游离DNA SEPT9甲基化与结直肠癌不同病理类型的相关性

目的研究血浆游离DNA中SEPT9甲基化在结直肠癌(CRC)不同组织病理学类型、不同大体形态分型中的检出水平及临床意义。方法选取北京市肛肠医院术前检测SEPT9甲基化,术后进行病理学分析的大肠良恶性肿瘤209例,包括结直肠癌182例,腺瘤及神经内分泌瘤27例,统计SEPT9甲基化在结直肠癌不同组织学类型、不同大体形态分型的阳性检出率。结果SEPT9在不同组织学类型:腺癌(非特殊性)、腺癌(特殊型,如印戒细胞癌)、鳞癌、腺瘤、肠道其他肿瘤(主要为神经内分泌瘤、平滑肌瘤、间质瘤)中的阳性检出率分别为66.7%、63.6%、33.3%、4.5%和0；在不同大体形态分型:隆起型、溃疡型、隆起溃疡型中的阳性检出率分别为35.3%、50.0%和67.0%。结论SEPT9在不同组织学类型中阳性检出率不同,可能与大肠癌的发病机制及发病阶段有关。SEPT9在隆起型、溃疡型、溃疡隆起型肿瘤中阳性检出率依次升高,可能与肿瘤随着肿瘤进展,逐渐增大有关。

Relationship between SEPT9 methylation in plasma circulation DNA and different pathological types of colorectal cancer

To study the detection level and clinical significance of SEPT9 methylation in plasma free DNA in colorectal cancer (CRC) of different histopathological types and morphologic pathological types. Methods209 cases of colorectal benign and malignant tumors, including 182 cases of colorectal cancer, 27 cases of adenoma and neuroendocrine tumor, were selected from beijing anorectal hospital for preoperative detection of SEPT9 methylation and postoperative pathological analysis. ResultsThe positive rates of SEPT9 in different histological types:adenocarcinoma (nonspecific), adenocarcinoma (special type, such as signet ring cell carcinoma), squamous cell carcinoma, adenoma and other intestinal tumors (mainly neuroendocrine tumor, leiomyoma and stromal tumor) were 66.7%, 63.6%, 33.3%, 4.5% and 0, respectively; The positive detection rates in different morphological types:protuberant type, ulcerative type and protuberant ulcer type were 35.3%, 50.0% and 67.0% respectively. ConclusionThe positive rate of SEPT9 is different in different histological types, which may be related to the pathogenesis and stage of colorectal cancer. The positive detection rate of SEPT9 in tumor with protuberant type, ulcerative type and ulcerative protuberant type increased in turn, which may be related to the gradual increase of tumor with tumor progression.