储存时间对消毒后软式内镜微生物污染的影响

目的监测消毒后软式内镜在存放不同时间的微生物污染情况,以探讨软式内镜的安全储存期。方法采用便利抽样法选取重庆市某三甲医院内镜中心的27条软式内镜进行前瞻性研究,2017年10月—2019年2月,纳入消毒后内镜生物学监测合格的内镜,并常温下储存24、48、72、168 h,监测内镜管腔及表面菌落数及其合格率,同时监测镜柜空气与物体表面菌落数及其合格率。结果 108条次软式内镜分别储存24、48、72、168 h,内镜管腔与表面菌落合格率均为100%。其中108条次内镜储存24、48、72、168 h的表面菌落数为0;108条次内镜储存24、48、72 h的管腔菌落数为0,储存168 h 107条次内镜管腔菌落数为0,1条次内镜管腔菌落数为6 CFU/件,但无致病菌。镜柜空气与物体表面菌落数合格率均为100%。结论软式内镜高水平消毒合格后在合格环境下储存168 h是安全的,使用前可不必再消毒。

Effect of storage time on microbial contamination of flexible endoscope after disinfection

Objective To monitor the microbial contamination of flexible endoscopes at different storage time after disinfection, so as to explore the safe storage period of flexible endoscopes. Methods 27 flexible endoscopes were selected from the endoscopy center of a tertiary first-class hospital in Chongqing by convenient sampling, a prospective study was performed, from October 2017 to February 2019, endoscopes with qualified biological monitoring after disinfection were stored at room temperature for 24, 48, 72 and 168 hours, bacterial colonies and qualified rate of bacterial colonies in the lumen and surface of endoscopes as well as air and surface of endoscope cabinets were monitored. Results Qualified rates of bacterial colonies in lumen and surface of 108 times of endoscopes stored for 24, 48, 72 and 168 hours were all 100%, the number of surface colonies in 108 times of endoscopes stored for 24, 48, 72 and 168 hours were all 0; the number of lumen colonies in 108 times of endoscopes stored for 24, 48, and 72 hours were all 0; the number of lumen colonies in 107 times of endoscopes stored for 168 hours were all 0; the number of lumen colony in 1 endoscopes stored for 168 hours was 6 CFU/piece, but without pathogenic bacteria. Qualified rates of colonies in air and object surface of endoscope cabinets were both 100%. Conclusion It is safe to store flexible endoscope in the qualified environment for 168 hours after high level disinfection, and it is unnecessary to disinfect it before use.