热灭活对输血相关感染标志物的影响

 目的 探讨热灭活对血清和全血两种不同类型标本中输血相关感染标志物结果的影响。方法 收集乙型肝炎病毒表面抗原(HBsAg)、丙型肝炎病毒抗体(抗-HCV)、人类免疫缺陷病毒抗体(抗-HIV)、梅毒螺旋体抗体(抗-TP)、乙型肝炎病毒(HBV) DNA和丙型肝炎病毒(HCV) RNA阳性血清标本共57份,HBsAg、抗-HCV、抗-TP和HBV DNA阳性全血标本共38份。将标本56℃水浴加热30 min灭活(可有效灭活冠状病毒)处理后,采用化学发光和荧光定量聚合酶链式反应(PCR)分别检测相应的项目,比较热灭活前后各指标的差异。结果 血清灭活后HBsAg、抗-HCV、抗-HIV、抗-TP、HBV DNA和HCV RNA检测结果与灭活前相比,差异均无统计学意义(均P>0.05);全血灭活后HBsAg、抗-HCV、抗-TP和HBV DNA检测值与灭活前相比,差异亦均无统计学意义(均P>0.05);Bland-Altman散点图显示 所有的差值数据均位于95%一致性界限(95%LoA)内,各指标灭活后与灭活前存在较好的一致性。结论 56℃水浴加热 30 min的处理方法对输血相关感染标志物的检测无明显影响,在新型冠状病毒疫情期间可考虑推广使用。

Effect of heat inactivation on blood transfusion-related infection markers

Objective To explore the effect of heat inactivation on blood transfusion-related infection markers in serum and whole blood specimens. Methods A total of 57 positive serum specimens of hepatitis B virus surface antigen (HBsAg), anti-hepatitis C virus antibody (anti-HCV), anti-human immunodeficiency virus antibody (anti-HIV), anti-Treponema pallidum antibody (anti-TP), HBV DNA and HCV RNA as well as a total of 38 positive whole blood specimens of HBsAg, anti-HCV, anti-TP and HBV DNA were collected. After specimens were inactivated in a water bath at 56℃ for 30 minutes (which can effectively inactivate coronavirus), chemiluminescence and fluorescence quantitative polymerase chain reaction were adopted to detect the corresponding markers, differences among indicators before and after heat inactivation were compared. Results After serum was inactivated, detected results of HBsAg, anti-HCV, anti-HIV, anti-TP, HBV DNA and HCV RNA were not significantly different from those before inactivation (all P>0.05); after whole blood was inactivated, detection results of HBsAg, anti-HCV, anti-TP and HBV DNA were not statistically different from those before inactivation (all P>0.05); Bland-Altman scatterplot showed that all the difference data were within 95% limits of agreement (95% LoA), there was a good consistency in each indicator before and after inactivation. Conclusion The treatment method of heating at 56℃ water bath for 30 minutes has no obvious effect on the detection of blood transfusion-related infection markers, and can be widely used during the epidemic of coronavirus disease 2019.