| 喜树茎尖组织培养与植株再生 |
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| 引用本文： | 吕立堂,朱冬雪,赵德刚.喜树茎尖组织培养与植株再生[J].中草药,2004,35(6):682-684. |
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| 作者姓名： | 吕立堂 朱冬雪 赵德刚 |
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| 作者单位： | 贵州大学农业生物工程省级重点实验室,贵州,贵阳,550025 |
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| 基金项目： | 贵州省科学技术基金资助 ( 2 0 0 2 3 0 3 3 )，贵州省科技厅专项基金 (黔科合字 ( 2 0 0 3 ) JN0 12 ) |
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| 摘 要： | 目的探索喜树的人工快繁。方法以喜树幼苗的茎尖作为外植体，培养在附加不同激素的培养基上。结果以B5培养基附加6～BA 0．2mg／L。，IBA 0．05mg／L和AS(afenine sulfate，10mg／L)对丛生芽的诱导与增值效果最佳，而附加IBA 0．5mg／L，KT 0．1mg／L，和AS 10mg／L的生根效果最佳。试管苗移栽到珍珠岩-土壤(3：7)的基质中生长良好，成活率高达96％。结论为喜树的开发利用提供了一条新途径。
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| 关 键 词： | 喜树 植株再生 组织培养 |
| 文章编号： | 0253-2670(2004)05-0682-03 |
| 收稿时间： | 2003/9/10 0:00:00 |
Tissue culture of shoot-tip and plantlet regeneration of Camptotheca acumunata |
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| LU Li-tang,ZHU Dong-xue and ZHAO De-gang.Tissue culture of shoot-tip and plantlet regeneration of Camptotheca acumunata[J].Chinese Traditional and Herbal Drugs,2004,35(6):682-684. |
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| Authors: | LU Li-tang ZHU Dong-xue and ZHAO De-gang |
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| Institution: | Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou University, Guiyang 550025, China;Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou University, Guiyang 550025, China;Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou University, Guiyang 550025, China |
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| Abstract: | Object To explore the artificial propagation of the medicinal plant, Camptotheca acuminata Decne. Methods The shoot-tip was tested as the explants and cultured on culture media with different portions of hormone.Results The best medium for bud induction was the B5 basic medium with additions of 6-BA (0.2 mg/L), IBA (0.05 mg/L), and AS (afenine sulfate, 10 mg/L). While B5 with the additions of IBA (0.5 mg/L), KT (0.1 mg/L), and AS (10 mg/L) was suitable for rooting. The seedling was cultivated and grew well on the base material mixed with perlite-soil (3:7). The survival rate of transplant was up to 96%. Conclusion The above mentioned method provides a new effective way to exploit this plant resources. |
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| Keywords: | Camptotheca acuminataDecne plantlet regeneration tissue culture |
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