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| 生物素-亲合素斑点免疫金渗滤试验检测血清中登革病毒4型抗原 | |
| 引用本文： | 姚海军,曹虹,刘久来,段馥琴.生物素-亲合素斑点免疫金渗滤试验检测血清中登革病毒4型抗原[J].南方医科大学学报,1999,19(2):163-164. |
| 作者姓名： | 姚海军 曹虹 刘久来 段馥琴 |
| 作者单位： | 1. 广州市卫生防疫站病毒室,广州,510080 2. 第一军医大学微生物学教研室,广州,510515 3. 河南省卫辉市人民医院,卫辉,453100 |
| 摘 要： | 目的建立用斑点免疫金渗滤试验（ＤＩＧＦＡ）检测血请中登革病毒抗原的方法。方法一株ＤＶ４型特异性单克隆抗体点加于硝酸纤维膜上，以捕获血清中的ＤＶ４抗原。借助黄病毒组反应性单克隆抗体、生物素化革抗鼠ＩｇＧ抗体、金标ＳＰＡ和金标亲合素，建立用ＤＩＧＦＡ检测血清中登革病毒抗原的方法。结果常规ＤＩＧＦＡ检测的敏感性高于病毒分离，而生物素一亲合素ＤＩＧＦＡ（ＢＡ－ＤＩＧＦＡ）的敏感性又高于常规的ＤＩＧＦＡ。常规ＤＩＧＦＡ可检测到５、０ＴＣＩＤ５０的病毒抗原，ＢＡ－ＤＩＧＦＡ可检测到４３ＴＣＩＤ５０的病毒抗原。结论作者建立的ＢＡ－ＤＩＧＦＡ简便、快速、敏感、特异，可用于快速诊断登革病毒４型感染。 |
| 关 键 词： | 登革病毒 斑点免疫金渗滤试验(DIGFA) 病毒分离 |
| 修稿时间： | 1998-10-02） |
Detection of dengue-4 virus antigens in serum by biotin-avidin dot immunogold filtration assay |
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| Yao Haijun,Cao Hong,Liu Jiulai,Duan Fuqin.Detection of dengue-4 virus antigens in serum by biotin-avidin dot immunogold filtration assay[J].Journal of Southern Medical University,1999,19(2):163-164. | |
| Authors: | Yao Haijun Cao Hong Liu Jiulai Duan Fuqin |
| Abstract: | Objective to develop dot-immunogold filtration assay (DIGFA)todetect dengue virus antigens in serum .Methods One dengue-4 virus type-specific monoclonal antibody was fixed on nitrocellulose membranes to capture dengue-4 virus antigens in serum, With thi telp of flavivrus grop-reactive monoclonal antibody, biotintlated goat anti-mouse lgG antibody,collidal gold labeled SPA and avidin ,DIGFA to detect dengue-4 antigens in serum was developed Results routine DIGFA was more sensitive than virus isolation, and biotin-avidin DIGFA(BA-DIGFA)was more sensitive than rotine DIGFA the sensitivity limits of routine digfa and BA-DIGFA was 5.0 and TCID50 respectively,Conclusion the BA-DIGFA is simple, rapid ,sensitive, specific for detecting dengue virus in serum, and can be used to diagnose dengue-4 virus infection rapidly. |
| Keywords: | dengue virus dot-immunogold filtration assay(DIGFA) virus isolation |
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