大鼠肺缺血再灌注对肺组织乙醛脱氢酶2和醛类物质的影响

目的研究肺缺血再灌注（ischemia／reperfusion。I／R）对乙醛脱氢酶2（ALDm）活性和含量，丙二醛（MethaneI）icarboxylicAldehyde，MDA）含量及4羟壬烯醛（4-hydroxy-2·noncnal，4-HNE）含量的影响。方法选取36只250—350g的雄性sD大鼠，采用在体原位肺I／R损伤模型，根据缺血再灌注时间不同随机分为A组（空白对照组即sham组）、B组（平衡灌注15rain组）、c组（缺血停呼吸30rain组）、D组（缺血停呼吸60min组）、E组（再灌注恢复呼吸30min组）和F组（再灌注恢复呼吸60min组），每组6只。在观察结束后取肺组织检测ALDH2活性和含量，MDA和4．HNE含量。结果A组、B组、C组、D组、E组和F组ALDH2活性分别为（2．26±0．45，2．30±0．48，2．40±0．69，2．21±0．42，2．21±0．42和2．16±0．62），含量分别为（0．91±0．11，1．21±0．61，1．23±0．38，1．11±0．38，1．00±0．47和O．92±0．34）组间差异无统计学意义。D组、E组和F组大鼠肺MDA含量（11．03±0．51，12．10±0．29和11．73±0．57）较A组和B组（10．35±0．52和10．30±0．56）升高，差异有统计学意义（P〈0．05）；E组和F组大鼠肺MDA含量高于c组（10．87±0．62）和D组，差异有统计学意义（P〈0．05）；其他各组之间差异无统计学意义。D组、E组和F组大鼠肺4-HNE含量（2．15±0．09，2．81±0．22和2．76±0．31）较A组和B组（1．93±0．1l和1．97±0．10）升高，差异有统计学意义（P〈0．05）；E组和F组大鼠肺4-HNE含量高于c组（2．05±0．08）和D组，差异有统计学意义（P〈0．05）；其他各组之间差异无统计学意义。结论随着缺血时间的延长，肺中醛类物质（MDA和4．HNE）开始蓄积，再灌注过程中醛类物质继续升高，ALDH2活性和含量未受到明显抑制

The Influence of Rat Lung Ischemia/Reperfusion on Aldehyde Dehydrogenases 2 and Aldehydes

Objective To study The influence of Lung ischemia/reperfusion（I/R） on Aldehyde dehydrogenases 2＂s （ALDH2） activity and content, as well as its＂ influence on Methane Dicarboxylic Aldehyde（MDA） and 4-hydroxy-2-nonenal＇s（4-HNE） content. Methods 36 SDmale rats weight 250-350g were randomly divided into group A （sham group）, group B （Balance perfusion 15mln group）, group C （ischemia 30min group）, group D（ischemia 60rain group）, group E（reperfusion 30rain group）, group F （reperfusion 60min group）. Each group has 6 rats.The model we established in this study is the in situ isolated-perfused lung model. Lung tissues were collected in different times and the ALDH2 activity and content, MDA content and 4-HNE content were detected. Results There is no significant difference in A,B,C,D,E and F groupin the activity of ALDH2 （2.26±0.45, 2.30±48, 2.40±0.69, 2.21±0.42, 2.21±0.42 and 2.16±0.62） and the content ofALDH2 （0.91±0.11,1.21±0.61,1.23 ±0.38,1.11±0.38, 1.00±O.47 and 0.92±0.34）. Compared with group A and B （10.35±0.52and 10.30i-0.56）, the level of MDA were significantly increased ingroup D, group E and group F （11.03±0.51, 12.10±0.29 and 11.73±0.57） （P〈0.05）; compared with groupC（10.87±0.62） and group D, the level of MDA were significantly increased ingroup E and group F （P〈0.05）; There is no significant difference in the level of MDA between other groups. Compared with group A and B （1.93±0.11 and 1.97±0.10）, the level of 4-HNE were significantly increased ingroup D, group E and group F （2.15±0.09, 2.8 ±0.22and 2.76±0.31） （P〈0.05）;compared with group C（2.05±0.08） and group D, the level of 4-HNE were significantly increased ingroup E and group F（P〈0.05）; There is no significant difference in the level of 4-HNE between other groups. Conclusion As the extension of ischemia time, aldehydes（MDA and 4 - HNE） in the lung began to accumulate and continue to rise in the process of reperfusion, the activity and content of ALDH2 was not significantly inhibited.