In situ hybridization localization of choline acetyltransferase mRNA in adult rat brain and spinal cord |
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| Affiliation: | 1. Department of Anatomy and Neurobiology, University of California, Irvine, CA 92717 USA;2. Department of Biochemistry and Molecular Biology, Mayo Clinic, Jacksonville, FL 32224 USA;1. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland;2. Bracco Suisse S.A., Plan-les-Ouates, Geneva, Switzerland;1. Laboratory of Behavioral Neuroscience, National Institute on Aging (NIA), National Institutes of Health (NIH), Baltimore, MD, USA;2. Clinical and Translational Neuroscience Unit, Laboratory of Behavioral Neuroscience, National Institute on Aging (NIA), National Institutes of Health (NIH), Baltimore, MD, USA;3. HiThru Analytics, Laurel, MD, USA;4. Department of Biostatistical Sciences, Wake Forest University School of Medicine, Winston-Salem, NC, USA;5. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA;6. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA;7. Translational Research and Medical Services Section, National Institute on Aging (NIA), National Institutes of Health (NIH), Baltimore, MD, USA;8. Laboratory of Clinical Investigation, National Institute on Aging (NIA), National Institutes of Health (NIH), Baltimore, MD, USA;9. Longitudinal Studies Section, National Institute on Aging (NIA), National Institutes of Health (NIH), Baltimore, MD, USA;10. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA;11. Department of Neurology, Emory University School of Medicine, Atlanta, GA, USA;12. Institute of Pharmaceutical Science, Kings College London, London, United Kingdom;13. Department of Neurology, Duke University School of Medicine, Durham, NC, USA;1. Department of Neuroscience, Columbia University, New York, NY 10032, USA;2. Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA;3. New York State Psychiatric Institute, New York, NY 10032, USA;4. Biomedical Research Foundation of the Academy of Athens, 115 27 Athens, Greece;5. Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA;6. Department of Psychiatry, Columbia University, New York, NY 10032, USA;7. Division of Systems Neuroscience, New York State Psychiatric Institute (NYSPI)/Research Foundation for Mental Hygiene, Inc. (RFMH), New York, NY 10032, USA;8. Kavli Institute for Brain Science, Columbia University, New York, NY 10032, USA;9. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10032, USA;1. INSERM, U1148, Laboratory for Vascular Translational Science, X. Bichat Hospital, 46 rue Henri Huchard, 75018, Paris, France;2. Paris Diderot University, Paris 13 University, Sorbonne Paris Cité, Paris, France;3. FRIM, INSERM UMS 034 Paris Diderot University, X. Bichat Hospital, 75018, Paris, France;1. Case Western Reserve University, Department of Biomedical Engineering, Cleveland, OH 44106, USA;2. Cleveland Clinic Foundation, Department of Cellular and Molecular Medicine, Cleveland, OH 44195, USA;3. Hathaway Brown School, Shaker Heights, OH 44122, USA |
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| Abstract: | The cellular distribution of choline acetyltransferase (ChAT) mRNA within the adult rat central nervous system was evaluated using in situ hybridization. In forebrain, hybridization of a 35S-labeled rat ChAT cRNA densely labeled neurons in the well-characterized basal forebrain cholinergic system including the medial septal nucleus, diagonal bands of Broca, nucleus basalis of Meynert and substantia innominata, as well as in the striatum, ventral pallidum, and olfactory tubercle. A small number of lightly labeled neurons were distributed throughout neocortex, primarily in superficial layers. No cellular labeling was detected in hippocampus. In the diencephalon, dense hybridization labeled neurons in the ventral aspect of the medial habenular nucleus whereas cells in the lateral hypothalamic area and supramammillary region were more lightly labeled. Hybridization was most dense in neurons of the motor and autonomic cranial nerve nuclei including the oculomotor, Edinger-Westphal, and trochlear nuclei of the midbrain, the abducens, superior salivatory, trigeminal, facial and accessory facial nuclei of the pons, and the hypoglossal, vagus, and solitary nuclei and nucleus ambiguus of the medulla. In addition, numerous cells in the pedunculopontine and laterodorsal tegmental nuclei, the ventral nucleus of the lateral lemniscus, the medial and lateral divisions of the parabrachial nucleus, and the medial and lateral superior olive were labeled. Occasional labeled neurons were distributed in the giantocellular, intermediate, and parvocellular reticular nuclei, and the raphe magnus nucleus. In the medulla, light to moderately densely labeled cells were scattered in the nucleus of Probst's bundle, the medial vestibular nucleus, the lateral reticular nucleus, and the raphe obscurus nucleus. In spinal cord, the cRNA densely labeled motor neurons of the ventral horn, and cells in the intermediolateral column, surrounding the central canal, and in the spinal accessory nucleus. These results are in good agreement with reports of the immunohistochemical localization of ChAT and provide further evidence that cholinergic neurons are present within neocortex but not hippocampus. |
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