过度运动对大鼠肾损伤敏感标志物的影响及人参总皂苷的干预研究

目的：探讨过度运动对大鼠肾组织及肾损伤敏感标志物的影响,及人参总皂苷的防治作用。方法：SD大鼠随机分为对照组（CTL）、过度运动组（M2、M24两个亚组）,人参总皂苷组（G2、G24两个亚组）,每组7只。采用大鼠运动跑台建立过度运动致大鼠急性肾损伤模型,人参总皂苷组于运动前1周给予人参总皂苷100mg·kg-1·d-1灌胃,模型组和对照组给予等体积蒸馏水灌胃,分别于运动后2h,24h检测尿蛋白、中性粒细胞明胶酶载脂蛋白（neutrophil gelatinase-associated lipocalin,NGAL）、N-乙酰β-D氨基葡萄糖苷酶（NAG酶）,腹主动脉采血检测肾功能变化,检测血浆及肾组织中血管紧张素Ⅱ（AngⅡ）浓度、SOD活性和MDA含量;光镜下观察肾组织病理改变。结果：过度运动可引起大鼠尿蛋白、尿NAG酶和血尿素氮、血NGAL水平升高,血浆及肾组织中AngⅡ浓度升高;伴随血清及肾组织中SOD活性降低、MDA含量升高,病理学光镜可见少量肾小管上皮细胞肿胀和脱落。人参总皂苷可明显降低大鼠过度运动后尿素氮水平,减少过度运动后蛋白尿和尿NAG酶排泄,抑制运动后肾组织AngⅡ升高,升高血清及肾组织中SOD活性,降低MDA含量（P〈0.05）,改善运动后肾脏病理损伤。结论：过度运动可引起尿蛋白及尿酶升高,及肾小管损伤;人参总皂苷对大鼠过度运动后AKI具有预防保护作用,其机制可能与抗氧化应激及减少运动后肾组织AngⅡ含量有关。

Excessive-exercise Induced Kidney Injury Sensitive Biomarker Change in Rats and the Effects of Ginsenoside

Objective:We investigate the changes of some sensitive biomarkers in rats undertaking excessive-exercise,and the effects of Ginsenoside (GS) which was a product from a plant herb-Ginsen.Methods:Male Sprague-Dawley rats divided into five groups: control group(CTL),over-exercise group( two sub-group: M2 and M24);Ginsenoside group ( two sub-group: G2 and G24).The animal model of excessive-exercise induced AKI was established by exhaustively running on the treadmill according protocol.We administrated with GS (...