| 纳络酮注射液对一氧化碳中毒迟发性脑病大鼠模型细胞凋亡及Caspase-3表达的影响 |
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| 引用本文： | 徐博,姬新才,吴华,孙亮.纳络酮注射液对一氧化碳中毒迟发性脑病大鼠模型细胞凋亡及Caspase-3表达的影响[J].陕西医学杂志,2008,37(8). |
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| 作者姓名： | 徐博 姬新才 吴华 孙亮 |
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| 作者单位： | 1. 陕西省人民医院,西安,710068
2. 陕西省疾病预防控制中心 |
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| 摘 要： | 目的:探讨纳络酮注射液对一氧化碳中毒迟发性脑病大鼠模型细胞凋亡及Caspase-3表达的影响。方法:将30只大鼠随机分为正常对照组、一氧化碳中毒迟发性脑病大鼠模型对照组和纳络酮注射液治疗组,原位末端标记法(TUNEL)检测细胞凋亡,免疫组化法检测Caspase-3的表达。结果:治疗组大鼠脑细胞凋亡率及Caspase-3表达较迟发性脑病对照组显著降低(P<0.05)。结论:纳络酮注射液对一氧化碳中毒迟发性脑病的脑保护作用机制可能与干预脑细胞凋亡相关基因表达并减少神经细胞凋亡有关。
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| 关 键 词： | 一氧化碳中毒/并发症 脑损伤 细胞凋亡 @Caspase-3 动物 实验 大鼠 |
Effects of Naloxone Injection on cell apoptosis and the expression of easpase-3 in delayed encephalopathy rat model of carbon monoxide poisoning |
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| Xu Bo Ji Xincai Wu Hua et alShaanxi Provincial People's Hospital.Effects of Naloxone Injection on cell apoptosis and the expression of easpase-3 in delayed encephalopathy rat model of carbon monoxide poisoning[J].Shaanxi Medical Journal,2008,37(8). |
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| Authors: | Xu Bo Ji Xincai Wu Hua Shaanxi Provincial People's Hospital |
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| Institution: | Xi'an 710068 |
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| Abstract: | Objective:To observe the effects of Naloxone injection on cell apoptosis and the expression of caspase-3 in delayed encephalopathy rat model of carbon monoxide poisoning.Methods:Thirty rats were randomly divided into three groups is normal group,delayed encephalopathy rate model of carbon monoxide poisoning control group and Naloxone injection treatment group.The cell apoptosis was detected by TUNEL stacning.The expression of caspase-3 was determined by immunohistochemical staining.Results:The cell apoptosis rate and the positive cell number of caspase-3 were significantly lower(P<0.05)in Naloxone injection treatment group than those in the delayed encephalopathy after carbon monoxide poisoning control group.Conclusion:The protective effect of Naloxone injection on the cerebral tissue after carbon monoxide poisoning may be related with its intervening in the expression of apoptosis-related gene and decreasing the degree of nerve cell apoptosis. |
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| Keywords: | Carbon nonoxide poisoning/complications Brain injuries Apoptosis @Caspase-3 Animals laboratory Rats |
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