| 核苷酸切除修复基因XPD反义RNA表达载体的构建及功能研究 |
| |
| 引用本文： | 蒋易,周李承,吴晓明,周宜开,雷于生,任恕. 核苷酸切除修复基因XPD反义RNA表达载体的构建及功能研究[J]. 华中科技大学学报(医学版), 2003, 32(3): 242-245 |
| |
| 作者姓名： | 蒋易 周李承 吴晓明 周宜开 雷于生 任恕 |
| |
| 作者单位： | 华中科技大学同济医学院公共卫生学院环境医学研究所,武汉,430030 |
| |
| 基金项目： | 国家自然科学基金重大项目 (No .39990 5 70 ) |
| |
| 摘 要： | 目的 构建核苷酸切除修复基因XPD反义RNA表达载体，初步探讨其对肺癌细胞DNA损伤修复能力及药物敏感性的影响。方法 通过RT-PCR技术获取XPD cDNA(2-667bp)片段并反向插入真核7表达载体反义载体pcDNA3．1，采用脂质体法将构建的载体质粒转染肺癌细胞株A549，经G418筛选，采用抗肿瘤药物阿霉素、顺铂对细胞进行染毒干预，干预结果采用SCGE和MTT综合判定。结果 转染细胞XPD mRNA表达受到抑制。单细胞凝胶电泳显示转染细胞DNA损伤修复能力降低。MTT法显示转染细胞药物敏感性增高。结论 XPD反义RNA能降低肺癌细胞DNA损伤修复能力，其为核苷酸切除修复功能的研究提供了一个新的方式。
|
| 关 键 词： | 核苷酸切除修复 XPD基因 反义核糖核酸 药物敏感性 |
Construction of Nucleotide Excision Repair Gene XPD Anti-sense RNA Expression Plasmid and Study on Its Functions |
| |
| Jiang Yi,Zhou Licheng,Wu Xiaoming et al Institute of Environmental Medicine,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan. Construction of Nucleotide Excision Repair Gene XPD Anti-sense RNA Expression Plasmid and Study on Its Functions[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2003, 32(3): 242-245 |
| |
| Authors: | Jiang Yi Zhou Licheng Wu Xiaoming et al Institute of Environmental Medicine School of Public Health Tongji Medical College Huazhong University of Science Technology Wuhan |
| |
| Affiliation: | Jiang Yi,Zhou Licheng,Wu Xiaoming et al Institute of Environmental Medicine,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
| |
| Abstract: | |
| |
| Keywords: | nucleotide excision repair gene XPD anti-sense RNA DNA repair capacity drug sensitivity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
