记忆性CD4+T淋巴细胞对小鼠腹腔移植心脏排斥反应的影响

目的 探讨记忆性CD4+T淋巴细胞对来自同一抗原特异性移植心脏急性排斥反应的影响.方法 将C57BL/6小鼠的皮肤移植给Balb/c小鼠,使其致敏,3个月后,取受者的脾脏,应用小鼠记忆性CD4+T淋巴细胞纯化试剂盒纯化记忆性CD4+T淋巴细胞.同时取未进行皮肤移植的Balb/c小鼠,同法获取非致敏CD4+T淋巴细胞.以C57BL/6小鼠为供者,正常Balb/c小鼠为受者,行腹腔心脏移植,移植前3周,实验组受者经阴茎背静脉注射记忆性CD4+T淋巴细胞;非致敏对照组受者经阴茎背静脉注射非致敏CD4+T淋巴细胞;空白对照组不注射任何T淋巴细胞.各组均于移植前1 d、移植当天及移植后1 d给予环孢素A.术后记录移植心脏存活时间;取移植心脏,进行病理学观察.结果 实验组、非致敏对照组和空白对照组移植心脏存活时间分别为(8.5±1.5)d、(25.7±5.5)d和(21.2±9.2)d,实验组明显短于另两组(P<0.05).移植心脏急性排斥反应的病理学分级,实验组为3.43±0.68,非致敏对照组为1.29±0.46,空白对照组为1.31±0.49,实验组病理学分级明显高于非致敏对照组和空白对照组(P<(0.05).结论 当记忆性CIN+T淋巴细胞再次接触同一抗原特异性的移植心脏时,可促进急性排斥反应的发生,且对常规剂量的环孢素A不敏感.

The effect of memorial CD4+T cells on ectopic heart acute rejection in abdominal cavity of mice

Objective To explore the effect of memorial CD4+T cells on acute rejection of the same antigenic specificity graft heart in mice.Methods The skin of mice from the C57BL/6 mice was transplanted to the Balb/c mice for sensitization,and 3 months later the spleen of receptor was obtained. The memorial CD4+ T cells were purified by the depuration kit of memorial CD4+ T cells in mice.Meanwhile,the naive CD4+ T cells of the Balb/c mice not subject to skin transplantation by the samc method were obtained.The normal Balb/c mice and the C57BL/6 mice were taken as the recipients and the donors.receiving heart transplantation in abdominal cavity of mice.In experimental group,memorial CD4+ T cells were injected into the mice,and in control group,naive CD4+ T cells were injected.In blank group,no T cells were Injccted.At first day after transplantation,cyclosporine A(5tion was carried out.Results The survival time of the heart allografts in experimental group,control group and blank group was(8.5±1.5),(25.7±5.5)and(21.2±9.2)days respectively.The survival time in experimental group was significantly shorter than in other two group(P＜0.05).The histological grade of heart allografts in experimental group.control group and blank group was 3.43±0.68,1.29±0.46 and 1.31±0.49 respectively.The histological grade of heart allografts in experimental group was significantly higher than in control group and blank group(P＜0.05).Conclusion Memorial CD4+ T cells can cause acute rejection when it contacts again homogeneity variant implanted heart,and the rejection is insensitive to cyclosporine A of common dose.