复合BMP同种异体半关节异位植入的免疫研究

目的探讨B M P骨诱导与免疫反应之间的关系。方法将复合B M P5m g的冻干异体半关节植入兔腰大肌肌袋内,并采用原位杂交技术对术后不同时间各组IL-1和IL-6m R N A表达强度进行比较。结果骨移植术后,实验组较对照组成骨作用增强。植骨局部在移植术后第4周起即可见IL-1和IL-6m R N A的阳性表达,实验组与对照组植骨局部均可见IL-1和IL-6m R N A的阳性表达,不同时间点各组间IL-1和IL-6m R-N A表达强度无明显差异。结论B M P作为外源性介质,在异位发挥诱导成骨作用的同时并不加重免疫排斥反应。

Study on immunity of hemi joint compounded with bone morphogenetic protein allograft heterotopic transplantation

Objective To investigate correlation between osteogenic induction and cellular immunogenic modulation of bone morphogenetic proteins (BMPs). Metho ds 5 mg of BMP from bone-matrix combined with lyophilized semi-joint allograft was embedded in the psoas muscle in 20 New Zealand rabbits, which were sacrific ed at 4, 6, 8, 10, 12 weeks after bone transplantation. Histological observation and in situ hybridization of IL-1, IL-6 with their cDNA probes were performed to compare their expression intensity. Results The capacity of bone formation w as enhanced more in the test group than in the control group. The IL-1 and IL- 6 mRNA expressions were observed both in the test and control groups but no sign ificant difference between the 2 groups was found. Conclusion The findings indic ate that exogenous BMPs do not increase the risk of immunological rejection in t he region of implanted bone allograft.