DEHP亚慢性暴露对大鼠精子运动参数影响

目的 观察邻苯二甲酸（2-乙基已基）酯（DEHP）染毒90 d对大鼠精子运动能力的毒性作用。方法 将健康清洁级SD雄性大鼠40只随机分为对照组和3个DEHP染毒组（5、50、500 mg/kg），进行90 d经口染毒；取睾丸、附睾称重，并用扩散法收集大鼠附睾尾精子，应用计算机辅助精子分析系统（CASA）对精子的运动参数进行检测。结果 5 mg/kg DEHP组大鼠精子直线性（LIN）为（44.00±2.52）%，明显低于对照组（50.00±2.71）%（P<0.05）；500 mg/kg DEHP组大鼠精子鞭打频率（BCF）为（5.8±4.2）Hz，明显低于对照组（10.4±1.7）Hz（P<0.05）；与对照组比较，500 mg/kg DEHP组大鼠精子的平均路径速度（VAP）、直线运动速度（VSL）、曲线运动速度（VCL）、精子头侧摆幅度（ALH）、BCF明显下降，差异均有统计学意义（均P<0.05）；随着DEHP染毒浓度增加，大鼠精子各项运动参数逐渐降低，精子的运动能力下降。结论 DEHP对大鼠附睾精子的运动能力有直接毒性作用。

Effect of low dose DEHP exposure on motility parameters of rat sperm in vitro

Objective To observe the toxic effect of 90 days' di-(2-ethylhexyl)phthalate (DEHP) exposure on motility of rat sperm.Methods Forty male Sprague-Dawley (SD) rats of clean grade were randomly divided into a control and a DEHP exposure group.The rats of the exposure group were treated with DEHP at the doses of 5,50 and 500 mg/kg orally for 90 days continuously.Testis and epididymis of all the rats were weighed and then the epididymis was collected with ‘diffusion’ method by the end of the treatment.Effects of DEHP on the motility of isolated sperms were analyzed with computer assisted sperm analysis (CASA) system.Results The percentage linearity(LIN)of the sperms of rats exposed to 5 mg/kg DEHP was significantly lower that those of the control rats (44.00±2.52% vs.50.00±2.71%,P<0.05).The beat cross frequency(BCF) of the sperms of rats exposed to 500 mg/kg DEHP was significantly lower that that of the control rats (5.8±4.2 Hz vs.10.4±1.7 Hz,P<0.05).All the motility parameters of sperms of the rats treated with 500 mg/kg DEHP reduced significantly compared with those of the controls (P< 0.05 or P< 0.01).With the increase of DEHP concentration,the parameters of sperm motility decreased.Conclusion The results demonstrate that DEHP exposure could result in direct toxic effects on motility of sperms in caudal epididymides of rats.