TRANSFER OF PLATELET-DERIVED GROWTH FACTOR-BB GENE BY GENE GUN INCREASES CONTRACTION OF COLLAGEN LATTICE BY FIBROBLASTS IN DIABETIC AND NON-DIABETIC HUMAN SKIN |
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| Authors: | Hiroaki Kuwahara Alfred T. Mitchell Michael D. MacKlin Jiangang Zhao Dmitry Listengarten Linda G. Phillips |
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| Affiliation: | 1. Department of Plastic and Reconstructive Surgery, Hokkaido University, Sapporo, Japan;2. Division of Plastic Surgery, The University of Texas Medical Branch, Galveston, Texas;3. Auragen Inc., Middleton, Wisconsin, USA |
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| Abstract: | We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGFBB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB. |
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| Keywords: | Gene Transfer Gene Gun Pdgf Fibroblast Diabetes |
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