黄芪总皂苷对H22荷瘤小鼠肿瘤抑制作用及机制

目的 探讨黄芪总皂苷对小鼠肝癌H₂₂肿瘤生长及免疫功能影响。方法 建立小鼠肝癌H₂₂移植瘤模型，小鼠随机分为对照组、模型组、黄芪总皂苷高、中、低剂量组；称重法检测小鼠抑瘤率、脾脏指数、胸腺指数；噻唑蓝法检测小鼠淋巴细胞增殖能力及T淋巴细胞转化能力，酶联免疫法检测小鼠血清中白细胞介素-2（IL-2）含量。结果 与对照组比较，模型组小鼠体重[（24.90±2.82）g]明显减轻，胸腺、脾脏指数[分别为（1.47±0.45）、（3.97±0.05）mg/g]明显下降，淋巴细胞增殖能力（0.10±0.02）及转化能力[（1.08±0.03）%]明显降低，血清IL-2含量[（87.59±28.60）pg/mL]明显下降（P<0.05）；与模型组比较，黄芪总皂苷各剂量组小鼠肿瘤生长均明显受到抑制，黄芪总皂苷低、中、高剂量组小鼠胸腺与脾脏指数[分别为（2.18±0.34/）、（2.47±0.54/）、（2.51±0.46/） 与（5.42±0.84）、（6.13±0.71）、（6.46±0.85）mg/g]升高，淋巴细胞增殖能力[分别为（0.17±0.01）、（0.18±0.03）、（0.19±0.01）] 和T淋巴细胞转化能力[分别为（1.14±0.07）、（1.15±0.06）、（1.20±0.07）%]增强，血清IL-2含量[分别为（117.67±30.28）、（121.80±34.04）、（124.93±37.10）pg/mL]升高。结论 黄芪总皂苷可抑制小鼠肝癌H₂₂肿瘤细胞的增殖，其机制可能与增强机体的免疫功能有关。

Inhibitive effect of total astragalus saponin on tumor and its mechanism in H22-bearing mice

Objective To investigate the effect of different concentration of total astragalus saponin on the growth of tumor cells and immune function in H₂₂-bearing mice.Methods The H₂₂ liver tumor model was established and the H₂₂-bearing mice were randomly divided into a tumor bearing model group,low-,medium-,and high-dose total astragalus saponin treatment groups;a control group was also set.At the end of 10 day's treatment,tumor inhibition ratio,spleen index,and thymus index of all the mice were determined with weighing method;multiplication capacity of lymphocytes and the transformation ability of T lymphocytes were tested with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay;meanwhile,interleukin-2 (IL-2) content was detected with enzyme-linked immunosorbent assay (ELISA).Results Compared with those in the control group,the body weight (24.90±2.82 g),thymus index (1.47±0.45 mg/g),and spleen index (3.97±0.05 mg/g) decreased significantly in the model group (P<0.05 for all); the lymphocyte multiplication capacity (0.10±0.02) and the T lymphocyte transformation ability (1.08±0.03) declined obviously in the model group (both P<0.01);the IL-2 content (87.59±28.60 pg/ml) was significantly down-regulated in the model group (P<0.01).Compared to that in the model group,the growth of tumor in total astragalus saponin treatment groups was obvious inhibited (P<0.01).For the mice of low-,medium-,and high-dose total astragalus saponin treatment groups,the thymus index (2.18±0.34,2.47±0.54,and 2.51±0.46 mg/g),the spleen index (1.14±0.07,6.13±0.71,and 6.46±0.85 mg/g),the capacity of lymphocyte multiplication (0.17±0.01,0.18±0.03,and 0.19±0.01),the lymphocyte transformation (5.42±0.84,1.15±0.06,and 1.20±0.07),and the content of IL-2 (117.67±30.28,121.80±34.04,and 124.93±37.10 pg/ml) were all higher than those of the model group.Conclusion The total astragalus saponin could inhibit the proliferation of tumor cells in H₂₂-bearing mice and the mechanism of the inhibitive effect may be related to increased immune function.