血络复欣颗粒对大鼠血栓形成及 小鼠肠系膜微循环障碍的影响

目的:研究血络复欣颗粒对大鼠颈动脉血栓形成及对小鼠肠系膜微循环障碍的影响。方法:取Wistar大鼠根据体重随机分为空白组、血络复欣组(生药5.2,10.4,20.8 g.kg-1.d-1)、复方血栓通组(0.4 g.kg-1.d-1)、曲克芦丁片组(48.6mg.kg-1.d-1),各组ig 7 d,末次给药后30 min将大鼠麻醉后固定,分离右侧颈总动脉,利用小动物血栓生成仪,在体观察并检测一定时间内电刺激导致的颈动脉血栓栓塞率;取昆明种小鼠根据体重随机分为空白组、血络复欣组(生药7.5,15.0,30.0 g.kg-1.d-1)、丹蒌片组(0.58 g.kg-1.d-1)、消旋山莨菪碱片组(3.9 mg.kg-1.d-1),各组ig 7 d,末次给药后30 min小鼠麻醉开腹,空肠袢局部滴加肾上腺素复制小鼠肠系膜微循环障碍模型,分别于滴加肾上腺素前后不同时间观察测量毛细血管管径大小、微血流速度和流态的变化情况。结果:血络复欣能显著降低电刺激导致的大鼠颈动脉血栓栓塞率;能够明显提高微动脉的血液流速、管径并改善流态。结论:血络复欣具有抑制血栓形成的作用;具有改善微循环作用。

Effect of XueluoFuxin Granula on Carotid Artery Thrombosis in Rats and Mesenteric Microcirculation Disturbance in Mice

Objective: To explore the effect of XueluoFuxin granula on carotid artery thrombosis in rats and mesenteric microcirculation disturbance in mice. Method: Wistar rats were randomly divided into six groups according to body weight: normal control group, XueluoFuxin groups (5.2, 10.4,20.8 g ·kg^-1 ·d^-1 crude herb), Fufang Xueshuantong group (0.4 g ·kg^-1 ·d^-1) and troxerutin group (48.6 mg ·kg^-1 ·d^-1). Rats were given drugs ig everyday for 7 days. The right common carotid arteries of anesthetized rats were separated to observe the thromboembolism events at 30 minutes after the last administration. Mice were randomly divided into normal control group, XueluoFuxin groups (7.5,15.0,30.0 g ·kg^-1 ·d^-1 crude herb), Danlou tablet group (0.58 g ·kg^-1 ·d^-1) and racanisodamine group (3.9 mg ·kg^-1 ·d^-1). Mice were given drugs ig for 7 days. Adrenaline was locally given on jejunal loops to induce mesenteric microcirculation disturbance at 30 min after the last administration. Micrangium diameter, blood flow rate in micrangium and hemorheology were observed. Result: XueluoFuxin reduced the thromboembolism events; accelerated the blood flow rate in micrangium and improved hemorheology. Conclusion: XueluoFuxin has the effects of inhibiting thrombosis and improving microcirculation.