参附益心颗粒对心衰大鼠心肌c-fos,c-myc表达的影响

目的:探讨参附益心颗粒对心衰大鼠心肌c-fos,c-myc表达的影响。方法:随机将150只雄性SD大鼠分为造模组130只,假手术组20只。造模组结扎左冠状动脉前降支,假手术组手术方法同上,仅在冠状动脉前降支下穿线不结扎。造模8周后,经超声测定大鼠左室射血分数(left ventricular ejection fraction,LVEF)<50%者随机分为5组(模型组、卡托普利组、氯沙坦组、参附益心颗粒(5.48,21.90 g.kg-1.d-1)2个剂量组,每组10只,另选假手术组大鼠10只。假手术组及模型组以纯净水ig,药物干预组将药物溶于纯净水中ig给药,每天1次,疗程4周。4周后超声测定LVEF,称重后,处死测左室重量指数(left ventricular mass index,LVMI),免疫组化法检测大鼠心肌中c-fos,c-myc表达。结果:21.90 g.kg-1.d-1参附益心颗粒可显著提高心衰大鼠LVEF,降低LVMI,抑制心衰大鼠心肌中c-fos,c-myc表达。结论:参附益心颗粒能够通过抑制心衰大鼠心肌中c-fos,c-myc表达,抑制心肌重塑。

Effect of Shenfu Yixin Granule on Heart Failure Rats Myocardial c-fos,c-myc Expression

Objective: To investigate the effect of the Shenfu Yixin Granule on heart failure rats myocardial c-fos,c-myc expression. Method: Hear failure rats were produced by left anterior descending coronary artery occlusion in one hundred thirty heathly male SD rats. Twenty rats obtained the surgery without ligation as sham control. After 8 weeks, rats with left ventricle ejection fraction(LVEF)<50% were randomly Yixin assigned to 5 groups (n=10 in each group): model group, captopril group, losartan group, two groups of Shenfu Yixin Granule(5.48,21.90 g ·kg^-1 ·d^-1). All the medicine was taken orrally once a day for 4 weeks. The rats in model group and sham control (n=10) were administered equivalent water. After 4 weeks, The LVEF was valued with Color Doppler Ultrasound again. The left ventricular mass index (LVMI) was checked. The expression of c-fos,c-myc were detected respectively by immune-histochemisty technique. Result: Shenfu Yixin Granule(21.90 g ·kg^-1 ·d^-1) increased the LVEF, decreased LVMI and inhibited the expression of c-fos,c-myc. Conclusion: Shenfu Yixin Granules inhibit the expression of c-fos,c-myc in heart failure rats.