沙苑子补肾固精的作用研究

目的:观察沙苑子对生精障碍动物的促生精作用及其对肾阳虚动物模型的影响。方法:通过建立生精障碍大鼠、小鼠模型及肾阳虚小鼠模型,计数生精障碍模型各组动物精子数量,观察各组精子的活力,测定血清性激素水平。测定肾阳虚模型各组小鼠肛温、游泳时间、自主活动、睾丸和精囊腺指数。结果:沙苑子提取物0.2,0.4 g.kg-1能明显增加生精障碍模型大鼠、小鼠的精子数、精子活动率及前列腺和精囊腺指数,能明显降低精子畸形数;能明显增加血清睾酮(T)含量,降低促黄体生成素(LH)、促卵泡生成素(FSH)水平;能明显增加肾阳虚模型小鼠的体温及自发活动次数、延长低温游泳存活时间及增加睾丸、精囊腺指数。结论:沙苑子提取物对生精障碍模型动物有显著的促生精作用,显著提高大鼠精子的质量,同时可改善血清性激素水平,对肾阳虚模型动物有明显的治疗作用。

Study of Astragali Complanati Semen on Tonifying Kidney for Arresting Spontaneous Emission

Objective: To observe the effects of Astragali Complanati Semen (ACS) on model animals with spermatogenic disturbance and deficiency of kidney yang. Method: The models of spermatogenic disturbance were established by feeding gossypol acetic acid (GAA) in rats and intraperitoneal injection of cyclophosphamide in mice, sperm quantities of animals in various groups were counted, sperm activities were observed, and the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T) were determined by radio immunoassay. The models of deficiency of kidney yang were established by feeding cortisone acetate. Rectal temperature, swimming time, spontaneous activity, testis weight, and seminal vesicle weight were measured. Result: SAC extract 0.2, 0.4 g ·kg^-1 markedly improved sperm quantity, sperm activity and the level of T in serum, decreased aberration rate of sperm and the level of LH and FSH in serum. It could markedly improve rectal temperature, swimming time, locomotor activity, testis index, and semina1 vesicle index. Conclusion: ACS extract can markedly promote the generation of sperm in spermatogenie disturbance animals, ameliorate serum sex hormone levels, increase the level of quality of sperm, and has remarkable therapeutic effect on deficiency of kidney yang in mice.