刺五加叶总黄酮和电针对大鼠脑缺血后Bcl-2蛋白表达的影响

目的:研究脑缺血与B细胞淋巴瘤/白血病-2(B cell lymphoma/leukemia-2,Bcl-2)蛋白的关系及刺五加总黄酮和针刺联合治疗作用。方法:雄性Wistar大鼠线栓大脑中动脉建立脑缺血动物模型,观察大鼠脑缺血后神经功能缺损评分;应用免疫组化法测定模型组(A组)、电针组(B组)、总黄酮组(C组)、针药结合组(D组)、假手术组(E组)、正常对照组(F组)Bcl-2的表达。结果:电针和刺五加总黄酮及其联合治疗组大鼠脑缺血后神经功能缺损评分显著低于单纯缺血组(P<0.01及P<0.05)。与E,F组比较,A,B,C,D组Bcl-2明显增加(P<0.01);与B,C组比较,D组表达明显增多(P<0.01及P<0.05)。结论:刺五加总黄酮和电针能提高Bcl-2蛋白表达,减少脑缺血区细胞凋亡的发生,尤其针药结合治疗有良好的疗效。

Influence of Total Flavones of Acanthopanacis Senticosi and Electroacupuncture on Expression of Protein Bcl-2 of Brain after Cerebral Ischemia in Rats

Objective: To study the relation between cerebral ischemia and Bcl-2(B cell lymphoma/leukemia-2,Bcl-2) and the effect of electroacupuncture and total flavones of Acanthopanacis Senticosi Folium for treatment. Method: Male Wistar rat model of cerebral ischemia was established with the method of thread middle cerebral artery occlusion.The rats were randomly divided into 6 groups: model control group( group A),acupuncture treatment group( group B),total flavones group( group C),acupuncture and Chinese medicine group( group D),sham operation group( group E)and normal control group( group F).The neurological deficit score was evaluated and immunohistochemistry was used to observe the expressions of Bcl-2. Result: Compared with those of the model group,the neurological deficit scores were significantly decreased in the treatment group( P <0.01, P <0.05).The expression of Bcl-2 increased markedly in the A,B,C and D groups( P <0.01).Compared treatment of acupuncture with Chinese medicine,group D was more obvious( P < 0.01, P <0.05). Conclusion: Electroacupuncture and total flavones of Acanthopanacis Senticosi Folium both can increase the expression of Bcl-2 and diminish apoptosis induced by ischemic cerebra,and electroacupuncture combined with Chinese medicine has significant effect.