中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤后的神经保护作用及血管内皮生长因子表达的影响

目的:观察中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤后神经保护作用及血管内皮生长因子(vascularendothelial growth factor,VEGF)表达的影响。方法:50只SD大鼠随即分成:假手术组,缺血再灌注组,脑缺血再灌注+中风皂贝化痰胶囊高、中、低剂量组(1.62,1.08,0.54 g.kg-1.d-1)。采用Zea-Longa方法制备大鼠脑缺血再灌注模型,观察中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤后的神经保护作用及VEGF表达的影响。结果:与假手术组相比,缺血再灌注大鼠出现严重的神经功能缺失症状,皮质和海马区VEGF表达增强;与脑缺血再灌注组相比,中风皂贝化痰胶囊治疗组能显著改善大鼠神经功能缺失症状,增加VEGF的表达,其中以中风皂贝化痰胶囊高剂量组的神经保护作用最为显著,VEGF阳性细胞区积分吸光度为(150.8±9.8)。结论:中风皂贝化痰胶囊对大鼠脑缺血再灌注损伤具有保护作用,其作用机制可能与增加VEGF的表达有关。

Influence of Zhongfeng Zaobei Huatan Jiaonang on Nerve Protection and Vascular Endothelial Growth Factor Expression in Cerebral Ischemia-Reperfusion Injury in Rats

Objective: To observe the effect of Zhongfeng Zaobei Huatan Jiaonang(ZFZBHTJN) on nerve protection and the VEGF expression in cerebral ischemia-reperfusion injury rats model. Method: Fifty SD rats were randomly divided into sham operation group, ischemia-reperfusion (I-R) group, ZFZBHTJN high, Medium, low dose group(1.62,1.08,0.54 g \5kg^-1 \5d^-1). The rat model of cerebral ischemia-reperfusion was produced by Zea-longa method. The impacts of ZFZBHTJN on neuro logic function and expression of VEGF were obseved. Result: Compared with sham operation group, I-R group presented severe neuro logic deficits and increases of VEGF expression in cortex and hippocampus.Compared with those in I-R group, neuro logic deficits of rats were relieved significantly and VEGF expression increased distinctly in ZFZBHTJN groups. High dose group (1.62 g \5kg^-1 \5d^-1) presented the most significant effect on nerve protection (P<0.01). Conclusion: ZFZBHTJN may relive cerebral ischemia-reperfusion injury in rats by increasing VEGF expression.