桂枝解郁方不同工艺抗抑郁作用的比较

目的:比较桂枝解郁方不同工艺的抗抑郁作用。方法:本实验将小鼠随机分成4组,即桂枝解郁方-A组、桂枝解郁方-B组(分别ig给予桂枝解郁液-A和桂枝解郁液-B 12.66 g.kg-1,每天1次,连续给药10 d)、盐酸阿米替林组(实验前3 dig给予盐酸阿米替林50 mg.kg-1,每天1次)、空白对照组(给予等体积的生理盐水)。采用小鼠强迫游泳实验、悬尾实验、自主活动实验和利血平拮抗实验,以小鼠不动时间、自主活动数和体温下降值为指标来评价桂枝解郁方不同工艺抗抑郁的作用。结果:在强迫游泳实验和悬尾实验中,桂枝解郁方-A能明显缩短小鼠的不动时间(P<0.05);在自主活动实验中,各组小鼠之间无显著性差异。利血平拮抗实验中,桂枝解郁方-A在4h能显著拮抗利血平诱发的小鼠体温降低(P<0.05)。结论:桂枝解郁方-A的抗抑郁作用优于桂枝解郁方B。

Comparison on Anti-depressant Effects of Guizhi Jieyu Fang by Different Techniques

Objective: To compare the anti-depressant effects of Guizhi Jieyu Fang by different techniques. Method: Mice were divided randomly into four groups: two treatment groups(Guizhi Jieyu Fang-A and Guizhi Jieyu Fang-B orally given for 10 days, 12.66 g ·kg^-1,daily),amitriptyline hydrochloride group(amitriptyline hydrochloride orally given for 3 days 50 g ·kg^-1,daily)and control group.The forced swimming test,tail suspension test,open field activity test and reserpine-induced hypothermia test were carried out to determine the antidepressant activity in mice.Mice immobility time in forced swimming test and tail suspension test,spontaneous activity frequency in open field activity test,and body temperature decrease in reserpine-induced hypothermia test were observed. Result: Guizhi Jieyu Fang-A could significantly shorten immobility time in forced swimming test and tail suspension test and inhabit the decrease of body temperature in reserpine antagonistic test at the 4^th hour(P<0.05),but no impact in open field activity test. Conclusion: Guizhi Jieyu Fang-A is better than Guizhi Jieyu Fang-B for anti-depression.