| 凉粉草总黄酮提取工艺优化 |
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| 引用本文： | 黄松,丁婕,郭云凤,赖小平. 凉粉草总黄酮提取工艺优化[J]. 中国实验方剂学杂志, 2011, 17(5): 5-7 |
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| 作者姓名： | 黄松 丁婕 郭云凤 赖小平 |
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| 作者单位： | 1. 广州中医药大学新药开发研究中心,广州,510006;东莞广州中医药大学中医药数理工程研究院,广东,东莞,523808
2. 广州中医药大学新药开发研究中心,广州,510006 |
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| 基金项目： | 国家自然基金重点项目，广东省科技厅中药现代化专项 |
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| 摘 要： | 目的:研究凉粉草中总黄酮的最佳提取工艺。方法:对凉粉草药材采用乙醇回流提取的方法,以槲皮苷为指标,利用分光光度仪测定总黄酮吸光度进行指标控制,采用L9(34)正交实验设计法筛选最佳提取工艺参数。结果:凉粉草总黄酮的最佳提取工艺为10倍量50%乙醇,回流提取2次,每次45 min。结论:本提取工艺方法简单、合理稳定,是凉粉草中总黄酮的最佳提取工艺。
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| 关 键 词： | 凉粉草 槲皮苷 总黄酮 提取工艺 |
| 收稿时间： | 2010-10-12 |
Study on Extracting Technology of Total Flavonoids from Mesona Herb |
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| HUANG Song,DING Jie,GUO Yun-feng and LAI Xiao-ping. Study on Extracting Technology of Total Flavonoids from Mesona Herb[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(5): 5-7 |
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| Authors: | HUANG Song DING Jie GUO Yun-feng LAI Xiao-ping |
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| Affiliation: | Deptartment of New Drug R&D in Guangzhou University of Chinese Medicine,Guangzhou 510006,China; Dongguan Institution for Mathematics and Theoretics Engineering Research,Guangzhou 523808,China;Deptartment of New Drug R&D in Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Deptartment of New Drug R&D in Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Deptartment of New Drug R&D in Guangzhou University of Chinese Medicine,Guangzhou 510006,China; Dongguan Institution for Mathematics and Theoretics Engineering Research,Guangzhou 523808,China |
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| Abstract: | Objective: To establish the extraction of the total flavonoids in Mesona chinemis. Method: The total flavonoids were extracted by refluxing.During this course,quercitrin was used as a index to control the abstraction.All the experiments were determined with UV.The best target was selected by L9(34) orthogonal test. Result: The best extract technology was to distill with 10 times 50% ethanol,refluxed 2 times and extracted for 45 min. Conclusion: The method is simple and feasible,and is the optimum extraction process of the total flavonoids from M.chinemis. |
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| Keywords: | Mesona chinemis quercitrin total flavonoids extraction process |
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