| 制何首乌多糖的脱蛋白研究 |
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| 引用本文： | 陈占科,王淑美,高珊,黎瑞汝,陈曦,苏智斌,梁生旺.制何首乌多糖的脱蛋白研究[J].中国实验方剂学杂志,2011,17(4):41-43. |
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| 作者姓名： | 陈占科 王淑美 高珊 黎瑞汝 陈曦 苏智斌 梁生旺 |
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| 作者单位： | 1. 河南中医学院,郑州,450008
2. 广东药学院,广州,510006 |
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| 摘 要： | 目的:对制何首乌多糖进行脱蛋白处理,筛选出最佳的脱蛋白方法。方法:以多糖损失率和蛋白去除率为衡量指标,选用了Sevag法、三氯乙酸法和酶法对制何首乌多糖提取物进行脱蛋白处理。结果:酶法、三氯乙酸法和Sevag法的蛋白去除率分别为87.62%,73.12%,62.65%,多糖损失率分别为8.13%,29.36%,20.24%。结论:酶法除蛋白是去除制何首乌多糖提取液中蛋白质的较理想方法。
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| 关 键 词： | 制何首乌 多糖 酶 脱蛋白 |
| 收稿时间： | 2010/9/28 0:00:00 |
Study on Deproteinization of Polysaccharide from Polygoni Multiflori Radix Preparata |
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| CHEN Zhan-ke,WANG Shu-mei,GAO Shan,LI Rui-ru,CHEN Xi,SU Zhi-bin and LIANG Sheng-wang.Study on Deproteinization of Polysaccharide from Polygoni Multiflori Radix Preparata[J].China Journal of Experimental Traditional Medical Formulae,2011,17(4):41-43. |
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| Authors: | CHEN Zhan-ke WANG Shu-mei GAO Shan LI Rui-ru CHEN Xi SU Zhi-bin and LIANG Sheng-wang |
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| Institution: | 1.He’nan University of Traditional Chinese Medicine,Zhengzhou 450008,China;2.Guangdong College of Phamarcy,Guangzhou 510006,China) |
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| Abstract: | Objective: To screen out the the optimal technology for deproteinization of polysaccharide from Radix Polygoni Multiflori Preparata. Method: The protein was removed by the enzyme,TCA and Sevage methods with the percentage of the removed protein (the PRP) and the decomposed polysaccharide (the PDP) as index. Result: The PRPs for the enzyme,TCA and Sevage methods were 87.62%,73.12% and 62.56%,the PDPs were 8.13%,29.36% and 20.24%. Conclusion: Enzymatic method of removing protein is an effective and alternative method. |
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| Keywords: | Radix Polygoni Multiflori preparata polysaccharide enzyme deproteinization |
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