黄连解毒汤对高脂血症大鼠血脂及氧自由基代谢的影响

目的:探讨黄连解毒汤(Huanglian Jiedu Decoction,HJD)对实验性高脂血症大鼠血脂及氧自由基代谢的影响。方法:将50只SD大鼠随机分为正常对照组,模型组,立普妥组(0.2 mg·kg-1),HJD低、高剂量组(6,12 g·kg-1)。除正常对照组外,其他组大鼠喂饲高脂饲料建立高脂血症模型。连续ig 8周后,测定血清总胆固醇(total cholesterol,TC)、三酰甘油(triacylglycerol,TAG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high-densitylipoprotein cholesterol,HDL-C)水平,以及超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化酶(glutathioneperoxidase,GSH-Px)活性及丙二醛(malondialdehyde,MDA)含量,并观察肝组织病理学改变。结果:与模型组比较,HJD各组能明显降低实验大鼠血清TC,TG,LDL-C,MDA含量,显著提高HDL-C含量和SOD,GSH-Px活性,并能减轻肝细胞脂肪变性。结论:HJD能有效调节高脂血症大鼠血脂代谢,提高机体抗氧化能力,改善肝组织病理损害。

Effects of Huanglian Jiedu Decoction on Blood Lipid and Oxygen Radical Metabolism in Experimental Hyperlipidemia Rats

Objective: To study the effects of Huanglian Jiedu Decoction (HJD) on blood lipid and oxygen radical metabolism in experimental hyperlipidemia rats. Method: Fifty SD rats were randomly divided into normal control group, untreated group, lipitor group(0.2 mg ·kg^-1), and low- and high-dose HJD group(6,12 g ·kg^-1). Except the normal control group, rats in the other groups were fed with high fat diet for 15 days to induce hyperlipidemia. Then the rats were administered with corresponding drugs for 8 weeks. After treatment, the serum levels of total cholesterol (TC), triacylglycerol (TAG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were assayed. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the level of malondialdehyde (MDA) were measured. The histological changes in liver tissues were observed. Result: Compared with the untreated group, HJD could significantly decrease the serum levels of TC, TG, LDL-C and MDA, increase the serum level of HDL-C and the activities of SOD and GSH-Px, and relieve the fatty degeneration of hepatic cells. Conclusion: HJD has the potential effect to regulate the lipid metabolism of hyperlipidemia rats, increase antioxidant activity and improve the histopathological injury of the liver.