丹参中总丹参酮的提取与纯化

目的:优选丹参中总丹参酮的提取纯化工艺条件。方法:采用正交设计法,以总丹参酮为含量测定指标,优选总丹参酮提取纯化工艺条件。以静态吸附及解析试验筛选最佳纯化树脂;通过动态吸附相关指标考察最佳洗脱条件。结果:最佳提取工艺为加8倍量90%乙醇,80℃水浴回流提取2次,每次1 h。最佳纯化工艺为采用D101型大孔吸附树脂,浓度40%样品以3 BV·h-1的流速吸附,用水5 BV去糖,以流速5 BV·h-1,90%乙醇洗脱6 BV。结论:优选工艺得到的总丹参酮含量较高,工艺简单,适宜工业化生产。

Study on Extraction and Purification Process of Total Tanshinone from Salvia miltiorrniza

Objective: To optimize a technique for extracting and purifying total tanshinones from Salvia miltiorrhiza. Method: Total tanshinones were selected as marker components and determined to optimize extraction and purification process of Salvia miltiorrhiza by orthogonal test. Result: The optimum extraction process was as follows: Salvia miltiorrhiza was added with 8 times alcohol of 90% and extracted 1 hour for 2 times. The optimum extraction purification was as follows: D101 macro porous resin was adopted in the experiment.The concentration of sample was 40%, the adsorptive speed of flow was 3 BV ·h^-1. The adsorbing column was eluted with 5 BV water to remove the sugar. Finally the adsorbing column was eluted with 6 BV 90% ethanol and the flow rate was 5 BV/h. Conclusion: The content of the total tanshinones obtained was high by the optimum purification conditions. The technique is simple and suitable for the modern production.