鹊肾树体外抗猴免疫缺陷病毒活性部位筛选

目的:筛选鹊肾树枝提取物中具有抗猴免疫缺陷病毒(SIV)的活性部位。方法:以SIV为靶病毒和相应的CEMx174细胞系统为模型,以3-叠氮胸苷(azidothymiding,AZT)为阳性对照药物,观察了鹊肾树枝的不同提取物对SIV的体外抑制作用;以试管法和滤纸法鉴定活性部位可能含有的化学成分。结果:鹊肾树枝的石油醚萃取物在62.5 mg.L-1时,对病毒抗原细胞抑制率为55.9%;乙酸乙酯萃取物在其浓度为15.625 mg.L-1时,对病毒抗原细胞抑制率为41.2%。石油醚萃取物可能含有挥发油、油脂和甾体或三萜类物质,而乙酸乙酯萃取物可能含有酚类物质。结论:鹊肾树枝中具有一定的抗SIV作用的成分可能为挥发油、油脂、甾体、三萜类或酚类物质。

Screening of Active Fractions of Streblus asper on Simian Immunodeficiency Virus

Objective: To screen the anti-simian immunodeficiency virus(SIV) active parts of the extracts of stem of Streblus asper . Method: The inhibitory effects of different extracts on SIV were observed when SIV and CEMx174 cell system were employed as the models and azidothymidine selected as the positive control drug. And chemical constituents of the anti-SIV active parts were identified by the test tube method and round filter paper. Result: The results showed that the inhibitory rates of SIV antigen positive cells were 55.9% and 41.2% when 62.5 mg ·L^-1 of petroleum ether extract and 15.625 mg ·L^-1 ethyl acetate extract of the stem of S. asper were used. The petroleum ether extract might contain essential oil, grease, steroidal or triterpene material, while the ethyl acetate extract contain phenolic compounds, indicating that the extract had certain inhibitory effect on SIV in vitro, but the other extract of the stem had no such effect. Conclusion: Essential oil, grease, steroidal and triterpene or phenolic compounds in the stem of S. asper may be the substance that has certain inhibitory effects on SIV.