水通道蛋白 mRNA表达与肺卫失宣大鼠肺损伤的 相关性及大黄的调节作用

目的:观察水通道蛋白(AQP1,AQP5)mRNA表达与肺卫失宣大鼠肺损伤的相关性及大黄的调节作用,探讨肺卫失宣的物质基础及大黄的保护机制。方法:以内毒素法建立肺卫失宣大鼠模型。60只大鼠随机分成正常对照组、肺卫失宣模型组、造模后5 d组、大黄预防(大黄颗粒剂9 g.kg-1d-1,ig连续2 d后造模)组和大黄治疗(造模后ig大黄颗粒剂9 g.kg-1d-1连续5 d)组。取肺组织做病理学检查并采用RT-PCR法检测肺组织AQP1,AQP5 mRNA的表达。结果:模型组大鼠肺组织肺水肿明显,大黄对肺水肿具有减轻作用。与正常对照组相比,模型组AQP1,AQP5 mRNA表达明显下降,造模后5 d组AQP1,AQP5 mRNA表达明显上调;与模型组比较,大黄预防组和治疗组AQP1,AQP5 mRNA表达明显上调;与造模后5 d组比较,大黄预防组和治疗组AQP1,AQP5 mRNA表达明显下调,预防组与治疗组表达无显著差异。结论:肺卫失宣大鼠可见肺水肿病理改变,AQP1,AQP5 mRNA表达异常(降低或异常高表达);大黄对肺卫失宣大鼠肺组织AQP1和AQP5 mRNA低表达或异常高表达有较强的改善作用,可能是其保护肺卫失宣肺损伤的重要作用机制之一。

Corelation between Expression of AQP1,AQP5 mRNA and Lung Injury in Rats with Lung-defensive Sluggishness Syndrome and Effect of Rhubarb on It

Objective: To observe the correlation between the expression of AQP₁,AQP₅ mRNA and the lung injury in the rats with lung sluggishness syndrome and the regulative effect of rhubarb on it, to explore the material foundation of the lung defensive sluggishness syndrom and the mechanism of rhubarb in the prevention and treatment of the lung injury. Method: Lipopolysaccharide(LPS) was injected into SD rats to perform lung-defensive sluggishness syndrome.Sixty rats was randomly divided into 5 groups:control group,lung-defensive sluggishness group,natural recovery after 5 days group,rhubarb prevention group (9 g ·kg^-1 ·d^-1 for 2 days before model establishment)and rhubarb treatment group(9 g ·kg^-1 ·d ^-1for 5 days after model establishment). Physiological and pathological staining was performed and real-time PCR was used to observe the expression of AQP₁,AQP₅ mRNA in the pulmonary tissue. Result: The pulmonary edema could be showed obviously in the lung-defensive sluggishness model and rhubarb could alleviate it. Compared with the control group,expression of AQP₁, AQP₅ mRNA of the model group was decreased,thus the natural recovery after 5 days group was elevated;no significantly difference can be seen between rhubarb prevention group and rhubarb treatment group. Conclusion: The pulmonary edema and abnormal expression of AQP₁,AQP₅ mRNA can be showed in lung-defensive sluggishness model and the rhubarb can inhibit them,which is one of the important mechanisms in protecting the lung injury of lung-defensive sluggishness syndrome.