开口箭脂肪酸成分气相色谱-质谱分析

目的:采用索氏提取法分别提取产自秦巴山区陕西汉中留坝和南郑两地的开口箭根茎中脂溶性成分。方法:经BF3-CH3 OH甲脂化处理后用气相色谱-质谱联用技术(GC-MC)分离和鉴定其成分,利用归一化法测定其各个成分的质量分数。结果:从产自秦岭南坡留坝的开口箭根茎中共分离鉴定出29种脂肪酸,主要种类有:正十六烷酸(42.815%),亚油酸(11.386%),反-油酸(6.378%),8,10-二甲氧基-十八烷酸(6.355%),正十八烷酸(4.18%),9,10,12-三甲氧基-十八烷酸(3.724%)等。从产自巴山北坡南郑的开口箭根茎中共鉴定出31种脂肪酸,主要种类有:正十六烷酸(32.404%),亚油酸(10.144%),正二十六烷酸(5.466%),8,10-二甲氧基-十八烷酸(5.418%),反-油酸(4.675%),正十八烷酸(4.664%),9,10,12-三甲氧基-十八烷酸(4.638%),9,11,13-三甲氧基-十八烷酸(4.422%),正二十二烷酸(4.234%)等。结论:秦巴山区产开口箭根茎中有多种重要脂肪酸,具有较高的营养保健价值,具有进一步开发利用的价值。

Analysis Fatty Acid Compositions of Tupistra chinensis by GC-MS

Objective: The fatty acid components from the rhizome of Tupistra chinensis produced in Hanzhong and Liuba of the Qinling-Daba mountains in Shaanxi province were extracted by soxhlet extraction. Method: The experiment were identified and determined by gas chromatography-mass spectrometry(GC-MS) after methyl esterification treatment. Result: The relative percentage of each component was confirmed by peak-area normalization method. Total 29 kinds of fatty acid were identified from T. chinensis produced in Liuba on Southern Slope of Qinling mountains. The main components of fatty acids were hexadecanoic acid(42.815%),9,12-octadecadienoic acid(11.386%),9-octadecenoic acid(6.378%),octadecanoic acid,8,10-dimethoxyl(6.355%),octadecanoic acid(4.18%),octadecanoic acid,9,10,12-trimethoxyl(3.724%),etc. Total 31 kinds of fatty acid were identified from T.chinensis in Nanzheng on Northern slope of Bashan mountain. The main components of fatty acids were hexadecanoic acid(32.404%),9,12-octadecadienoic acid(10.144%),hexacosanoic acid(5.466%),octadecanoic acid,8,10-dimethoxy(5.418%),9-octadecenoic acid(4.675%),octadecanoic acid(4.664%), octadecanoic acid,9,10,12-trimethoxyl(4.638%),octadecanoic acid, 9,11,13-trimethoxyl(4.422%),docosanoic acid(4.234%),etc. Conclusion: It was shown that there were some impotent fatty acids in T.chinensis which have potential development and application value in food nutrition and health care.