香椿子总多酚对心肌缺血再灌注大鼠的保护作用

目的:观察香椿子总多酚对心肌缺血再灌注大鼠的保护作用。方法:50只SD大鼠随机分成假手术组:冠脉下穿线不结扎+0.5%羧甲基纤维素钠、模型组:冠脉下穿线结扎+0.5%羧甲基纤维素钠、香椿子总多酚低剂量(XD,50 mg·kg-1)+冠脉下穿线结扎、中剂量(XZ,100 mg·kg-1)+冠脉下穿线结扎、高剂量(XG,200 mg·kg-1)+冠脉下穿线结扎组,各组均ig给药。采用左冠状动脉前降支结扎30 min再灌120 min的方法复制大鼠心肌缺血再灌注损伤模型。再灌注结束时取血清,测定血清中磷酸肌酸激酶(CK)、肌钙蛋白I(cTnI)、超氧化物歧化酶(SOD)、丙二醛(MDA)的水平。取出心脏,自结扎线以下平行均匀切成5片,用1%氯化三苯基四氮唑(TTC)浸染15 min,计算心肌梗死程度。结果:与模型组相比,香椿子总多酚处理组能有效降低心肌缺血再灌注大鼠血清中CK,cTnI,MDA的水平,增加SOD活力,减轻心肌梗死程度。结论:香椿子总多酚对心肌缺血再灌注大鼠具有一定的保护作用。

Protective Effects of Total Polyphenols Extracted from Toona sinensis on Myocardial Ischemia/reperfusion-induced Injury in Rats

Objective: To investigate the protective effects of total polyphenols extracted from Toona sinensis on myocardial ischemia/reperfusion-induced injury in rats. Method: Fifty SD rats were randomly divided into 5 groups: a sham operation group (Sham), a model group treated by ligation of the left coronary artery plus administration of 0.5% sodium carboxymethyl cellulose (Model), a group with model animals treated by intragastrical administration of a low dose of total polyphenols extracted from T. sinensis (50 mg ·kg^-1, XD), a group treated by a moderate dose (100 mg ·kg^-1, XZ), and a group treated by high dose (200 kg^-1 ·XG). Myocardial ischemia/reperfusion-induced injury was made by legating the left coronary artery (LCA) for 30 min followed by a 120 min reperfusion in anesthetized rats. After reperfusion, the levels of phosphocreatine kinase(CK), troponin I(cTnI), superoxide dismutase(SOD) and malondialdehyde(MDA) in serum were detected. Finally, the hearts of rats were cut into 5 pieces and stained by 1% TTC for 15 min; the degree of myocardial infarction was calculated. Result: Compared with the rats in model group, after administrated with polyphenols extracted from T. sinensis, the levels of CK, cTnT-I and MDA in serum were decreased, the activity of SOD was increased, and the severity of myocardial infarction was alleviated. Conclusion: Total polyphenols extracted from T. sinensis played a protective role in myocardial ischemia/reperfusion-induced injury.