比较电纺β-磷酸三钙/明胶引导组织再生膜与聚乳酸、聚丙交酯/乙交酯膜的生物相容性

背景:以往有很多学者通过发泡法将β-磷酸三钙与明胶复合得到引导组织再生材料,但将其采用静电纺丝技术制成纤维膜的报道很少.目的:制备新型引导组织再生膜,并比较其与聚乳酸,聚丙交酯/乙交酯膜的细胞毒性.方法:采用静电纺丝法制备β-磷酸三钙/明胶引导组织再生膜,通过扫描电子显微镜对纤维膜表面进行观察.通过MTT实验对电纺β-磷酸三钙/明胶膜与聚乳酸,聚丙交酯/乙交酯膜的细胞毒性进行对比.结果与结论:新型电纺β-磷酸三钙/明胶膜为多孔状,β-磷酸三钙颗粒呈结节状附着在明胶纤维表面,纤维直径平均为400～500 nm,分布比较集中,大多在200～500 nm之间.与聚乳酸,聚丙交酯/乙交酯膜及阴性对照之间细胞毒性差异无显著性意义(P>0.05).说明新型电纺β-磷酸三钙/明胶引导组织再生膜细胞毒性较低,基本符合生物材料安全性的要求,有望成为新型的引导组织再生膜材料之一.

Biocompatibility of electro-spinning beta-tricalcium phosphate/gelatin guided tissue regeneration menbrene versus polylactic acid and poly(lactide-co-glycolide) membrane

BACKGROUND: In the past, guided tissue regeneration materials were produced by 13-tricalcium phosphate (β-TCP) and gelatin (Gel), but the production of fiber membrane by electro-spinning technique was reported less.OBJECTIVE: To prepare a new kind of Gel with β-TCP hybrid nanofibrous membrane and testing its biocompatibility compared with polylactic acid (PLLA) and poly(lactide-co-glycolide) (PLGA) membrane.METHODS: The β-TCP/Gel guided tissue regeneration membrane was made by electro-spinning technique.Scanning electron microscopy (SEM) was employed to observe membrane surface.MTT test was performed to compare toxicity among β-TCP/Gel,PLLA, and PLGA membranes.RESULTS AND CONCLUSION: The β-TCP/Gel membrane was porous, and β-TCP granules were nodosity-adhered on surface of Gel.The average diameter of fiber was 500-600 nm.The distribution of fiber ranged from 200 to 500 nm.There was no significant difference in toxicity among PLLA, PLGA, and negative control group (P > 0.05).The results suggested that the β-TCP /Gel membrane was low cytotoxicity and suitable for tissue engineering.It would be a promising material for periodontal tissue regeneration.